Bradford法蛋白定量(BradfordProteinAssay)
Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within ......阅读全文
BIURET-PROTEIN-ASSAY
BIURET PROTEIN ASSAY MATERIALS Biuret Reagent Bovine serum albumin (BSA) Spectrophotometer and tubes PROCEDURE Prepare standard d
Bradford-protein-assay
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
Protein-Assay-(Spectrophotometer)
Protein Assay (Spectrophotometer)Use BSA (bovine serum albumin) 1mg/ml stock solution (1ml Eppendorf tubes) for standard curve.Place 0, 2, 5, 10, 15,
LOWRY-PROTEIN-ASSAY
The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-
Angiotensin-Protein-Kinase-Assay
James Hardwick's angiotensin assay protocol This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral
Biorad-Protein-Assay:-Bradford
Biorad Protein Assay: BradfordStandards: 1 mg/ml BSA stock- dilute 1:10 to get 0.1 mg/ml BSAAdd To get H-2O20 µl 2 µg/ml 780 µl40 µl 4 µg/ml 760 µl60
Bradford-Protein-Concentration-Assay
Bradford Protein Concentration Assayversion 01/07/2001Abbreviations:mcg = microgramsmcL = microlitersBSA = bovine serum albuminO.D. = optical densityd
In-Vitro-Protein-Ubiquitination-Assay
Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme (E1), ubiquiti
Use-of-the-Bradford-Protein-Assay-in-a-Microtiter-Plate-Format
Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change
Bradford法蛋白定量(Bradford-Protein-Assay-)
Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observ
Penicillan-Binding-Protein-Assay青霉素与细胞膜蛋白结合实验
Wash cells with 10 mM Tris pH 8French pressSlow speed spinHigh speed spinResuspend in 10 mM TrisSonicate 2 x 15 sec to remove ß-lacatamasesWash in 10
Antibody-Purification-using-Protein-A,-Protein-G,-or-Protein-L-Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should
Antibody-Purification-using-Protein-A,-Protein-G,-or-Protein-L-Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants主要试剂 Protein
Pectinase-assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
Protease-assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soft
Protease-assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part
DGK-Assay
Buffers: - 2X buffer 10 ml 0.5 M imidazol, pH 6.6 0.21 g LiCl 1.25 ml 1 M MgCl2 1.0 ml 0.1 M EGTA, pH 6.6 --> Bring volume up to 50 ml with distille
Phosphate-Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
TUNEL-assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Polygalacturonase-assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Aspartate-Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Motility-Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
Chemotaxis-Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Bradford-Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
Bradford-Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
MTT-Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
Radioiodination-of-protein
Radioiodination (by Jun Takagi,6/16/2000) Purpose and backgrounds Principle of radioiodination Addition of oxidizing reagents (such as chloram
Protein-Crystallization
Background:Proteins, like many molecules, can be prompted to form crystals when placed in the appropriate conditions. In order to crystallize a protei
Protein-Electrophoresis
DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh
Assay-of-Phospholipase-A-Activity
Phospholipases of the A type constitute a large family of esterases that catalyze the hydrolysis of the fatty acid ester bonds in phospholipids a