Measurement of ACTase activityNuclear magnetic resonance spect roscopy (NMR)The unique potential of NMR spectroscopy for monitoring simultaneously the concentrations of several metabolites in complex milieux makes it one of the most powerful techniques available to carry out this type of study.For NMR measurements, lysates or cell-free extracts were resuspended in 0.15 M NaCl constituted in 5:1 H2O/2H2O buffer mixtur......阅读全文
Different enzyme assays for ACTase study in H. pyloriACTase properties were studied in situ in cell-free extracts to obtain information on e
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helico