WIKI资讯

SYBRGreenQuantitativePCRProtocol

SummaryQuantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the threshold cycle (Ct) during PCR when the level of fluorescence gives signal over the background and is in the linear portion of the amplified curve. This Ct value is responsible for the accurate quantization of qPCR. SYBR Green is a dye that intercalates with doub......阅读全文