WIKI资讯
WIKI资讯
A variety of PCR additives and enhancing agents have been used to increase the yield, specificity and consistency of PCR reactions. Whilst these additives may have beneficial effects on some amplifications it is impossible to predict which agents will be useful in a particular context and therefore they must be empirically tested for each combination of template and primers. Some of the more popular of thes......阅读全文
· PCR Additives (Robert H. Cruickshank)A usr/localiety of PCR additives and enhancing agents
A variety of PCR additives and enhancing agents have been used to increase the yield, specificity and consistency of PCR reactions. Whilst these addit
PCR Additives (Robert H. Cruickshank)A usr/localiety of PCR additives and enhancing agents have been used to increase the yield, speci
实验概要protocal for PCR实验步骤PCR 1) Add the following to a microfuge tube: 10 ul reaction buffer
5. touchdown PCR 原理很简单,但的确是一个很有用的方法。举个例子就OK, ANNEALING TEMP. 55度94 5min 94 30s 60 30s 72 1min 2cycles&
PCR实验技巧增加PCR的特异性：1. prime理想的，只同目的序列两侧的单一序列而非其他序列退火的引物要符合下面的 一些条件a. 足够长,18-24bp,以保证特异性.当然不是说越长越好,太长的引物同样会降低特异性,并且降低产量b. GC% 40%~~~~60%c. 5'端和中间序列要多
增加PCR的特异性：1. primers design这是最重要的一步。理想的，只同目的序列两侧的单一序列而非其他序列退火的引物要符合下面的 一些条件a. 足够长,18-24bp,以保证特异性.当然不是说越长越好,太长的引物同样会降低特异性,并且降低产量b. GC% 40%~~~~60% c. 5&
增加PCR的特异性：1. primers design这是最重要的一步。理想的，只同目的序列两侧的单一序列而非其他序列退火的引物要符合下面的 一些条件a. 足够长,18-24bp,以保证特异性.当然不是说越长越好,太长的引物同样会降低特异性,并且降低产量b. GC% 40%~~~~60% c. 5&
Since PCR is often problematic on GC-rich templates, we have evaluated different PCR enhancing additives and generated an Combinatorial Enhancer Solut
PCRPolymerase Chain Reaction1) Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul templ
5. touchdown PCR 原理很简单,但的确是一个很有用的方法。举个例子，ANNEALING TEMP. 55度 94 5min 94 30s 60 30s 72 1min 2cycles 94 30s 59 30s 72 1min 2cycles 94 30s
PCR经验总结（先声明，此文不如分子克隆第三版权威与分子克隆相背处，请信分子克隆)一、增加PCR的特异性1、primers design这是最重要的一步。理想的，只同目的序列两侧的单一序列而非其他序列退火的引物要符合下面的一些条件1）足够长，18-24bp,以保证特异性。当然不是说越长越好，太长的引
· What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspect
What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not onl
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle Numbe
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
2020国际兽医检测诊断大会嘉宾新鲜出炉为进一步提高中国兽医诊断服务在可靠性、准确性、及时性和高通量等方面的表现，第二届国际兽医检测诊断大会将于2020年6月13-15日在南京国际展览中心举行。大会广泛邀请全球兽医检测诊断的杰出代表与不断进取的中国兽医进行深层次的分享、对话及相互启迪。一、大会详情时