免疫球蛋白提取技术(Immunoglobulinisolationtechnique)2
九、蛋白定量技术 (一)双缩脲测定法 1.原理 蛋白质中的肽键有双缩脲反应,在碱性溶液中与二价铜离子形成蓝紫色的络合物,在一定的范围内,颜色的深浅与蛋白质的含量成正比。此法特异性强,游离的氨基酸、小肽和核酸均不产生这种反应,但此法不够敏感,仅能测出毫克水平。 2.试剂配制 硫酸铜(CuSO4·5H2O) 1.50g 酒石酸钾钠  ......阅读全文
Isolation-of-papillary-cells
Isolation of renal papillary cells1. For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
DNA-isolation-extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolati
Isolation-of-murine-splenocytes
Overview In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the c
Isolation-of-Zebrafish-embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by keeping
Isolation-of-mouse-embryos
1. Sacrifice impregnated mouse. 2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps, use another to tear the mes
Isolation-of-colonic-epithelium
实验概要 The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine. First
Chromosomal-DNA-Isolation
Chromosomal DNA Isolation METHOD: Grow cells in 2-5 ml broth to late log phase. Pellet 1-2 ml cells in microfuge. Resuspend cells in 400
Cosmid-DNA-Isolation
实验概要 Isolation of high yields of highly pure cosmid DNA using PureLink™ HiPure Plasmid Purification Kits. 实验原理 The PureLink™ HiPure Plasmid Purif
Isolation-of-papillary-cells
实验概要 This protocols provides a general protocol for isolation of papillary cells. 实验步骤 Isolation of renal papillary cells 1. For isolation of pa
Isolation-Of-PCR-Products
实验概要 Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents. 实验原理 The ChargeSwitch® Tech
mice-islet-isolation
1. Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2. Which involves cannulation of the common bile duct and
RNA-Isolation-Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)
RNA-isolation-for-Microarray
Description RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure It is 3 days procedure.Day 1:1. Harvest the cells and cen
Arabidopsis-gDNA-isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
Isolation-of-liver-lymphocyte
Isolation of liver lymphocyte Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Isolation-of-bone-marrow
(contributed by Chris Jackson (chris.jackson@bris.ac.uk))A protocol I have used to isolate rat bone marrow is: 1. Kill the rat and dissect out the fem
Fungal-DNA-Isolation
Fungal DNA IsolationSaghai-Maroof MA, Soliman KM, Jorgensen RA, & Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species of C
Nucleolar-Isolation-Protocol
We recommend that you first download and read this page as a PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
Yeast-Nuclei-Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
Poly(A)+RNA-Isolation
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the
Isolation-of-colonic-epithelium
The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
RNA-Isolation-Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Isolation-of-lymphatic-endothelial-cells
Dermal Cell Suspensions 1. Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery.
Plasmid-isolation-from-yeast
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml b
Isolation-of-Microtubules-(Bovine-Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphi
T-and-B-cell-Isolation
Reagents Heparin - 1000 U/ml Ficoll-Hypaque PBS RPMI-1640 supplemented with 10 mM glutamine and 15% FBS AET (0.14M) Dissolve 1.967 g AET in 35 m
ISOLATION-OF-RNA-FROM-BACTEROIDS
3 g nodules (fresh or frozen in liquid N2) were ground to a powder in mortar and pestle with liquid N2. To the powder was added ice cold 0.5 M mannit
Lipoprotein-Isolation--First-week
As insects have an open circulatory system, the hemolymph can be simply collected through an incision in the body wall. Most conveniently, you should
Keratocyte-isolation-(corneal-fibroblasts)
Corneal keratocytes (corneal fibroblasts) that this corneal layer is specialized fibroblasts residing in the stroma, representing about 85-90% of
Cajal-Body-Isolation-Protocol
Buffers and solutions(All solutions are supplemented with Complete Protease inhibitor tablet (Roche, Cat no: 1-873-580) at the final concentration of