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Twodimensionalpeptidemapping

This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol. This was improved by Karen Beemon and Tony Hunter. Tamara Hurley and Kunxin Luo adapted it to proteins transferred to filters.Procedure:1. Immunoprecipitate ......阅读全文