WIKI资讯
WIKI资讯
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These cells must be competent. (Protocol for how to make competent cells.)a) Take competent cell stock aliquot (about 100 mL) out of -80oC freezer. Place on ice to thaw about 5 minutes.b) Take one mL of plasmid DNA (usually a pRSET B vector with your protein cloned inside DNA seq of pRSET B) and add to the thawed com......阅读全文
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia,
蛋白质提取和纯化（主要内容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
Protein Construct Expression and Purification Procedures (Gimila's Lab) Protein Expression (Mark's Lab) ·
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
实验概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as tran
Interaction Trap/Trap Two-Hybrid System· Yeast Two-Hybrid System (Finley Lab)This is one of
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke and Michael B. YaffeCenter for Cancer Research, Massachusetts Institute
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Pichia pastoris is a methylotropic yeast used to express high amounts of protein. Secreted expression is achieved with a cleavalable faktor. To yield&
· Yeast Two-Hybrid System (Finley Lab)This is one of the most comprehensive and detailed gui
实验概要 Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and g
实验概要The exponential amplification via reverse transcription polymerase chain reaction provides for a highly sensitive technique in which a