Amersham2DPAGE中文版

目录

第一章 样品制备                                                               2

1.1   一般性原则                                                                  2
1.2   样品制备程序                                                              3
1.2.1 培养细胞样品处理方法                                            3
1.2.2  组织样品处理方法                                                    3
1.2.3 文献报道较多的裂解液配方                                     4

第二章 第一向等电聚焦 （IEF）                                  7
2.1   IPG 胶条的水化和电泳                                             7
2.1.1 仪器                                                                            7
2.1.2 试剂                                                                            7
2.1.3 实验步骤                                                                    7
2.2   IPG胶条的平衡                                                           9
2.2.1 仪器                                                                          10
2.2.2 试剂                                                                          10
2.2.3 实验步骤                                                                  10

第三章 第二向 SDS 电泳                                                12
3.1   垂直 SDS-PAGE                                                     12
3.1.1 溶液                                                                          12
3.1.2 灌胶步骤                                                                  12
3.1.3 电泳步骤                                                                  14

第四章 2-DE 胶蛋白质点的检测                                     15
4.1   考马斯亮兰染色                                                        15
4.1.1 经典的考马斯亮兰染色程序                                   15
4.1.2  Neuhoff 胶体考染法                                                15
4.1.3 热考马斯亮兰染色及二次染色法                           16
4.2 硝酸银染色                                                                  16

Appendix I  Troubleshooting                                            18
Appendix II  Solutions                                                       23