| Company | Method | Detection Range | -Compatibility | Assay protocol | |
| Absorbance 280nm | 0.1-1 OD280nm/ml | Absorbance of aromatic amino-acids (Trp, Tyr and Phe at less extent). Only for purified proteins with known absorptivity factor (Use ExPASy ProtParam tool to inquire E1% 280nm) | Read absorbance 280nm. | Nucleic acid, detergents, cofactors, phenolic compounds, pigments, reducing agents, etc etc. | |
| AMERSHAM- BIOSCIENCE (PHARMACIA) (# 80-6483-56) | 2-D Quant Kit | 0–50 µg (1–50 µl) | Designed for the accurate determination of protein concentration in samples prepared for electrophoresis and presence of detergents, Urea, DTT, EDTA, Ampholites, etc, and many buffer components. | Quantitative precipitation of proteins while leaving interfering substances behind. | |
| BIO-RAD (#500-0001/2/6) | Bio-Rad Protein Assay (Modified Bradford) (pdf) | 0.2–0.9 mg/ml | Compatible with reducing agents (See list of compatible reagents on BioRad cataloge) | Minimum incubation time 15minutes. Assay wavelength 650-750nm | Detergents, basic buffers |
| BIO-RAD (#500-0111/2) | DC Protein Assay (Modified Lowry) (pdf) | 0.1–2.0 mg/ml | Compatible with detergents, basic buffers (See list of compatible reagents on BioRad cataloge) | Minimum incubation time 15minutes. Assay wavelength 595nm | Reducing agents |
| BIO-RAD(#500-0121/2) | RC DC Protein Assay (Modified Lowry) (pdf) | 0.1–2.0 mg/ml | Compatible with detergents, reducing agents, Laemmli sample buffer with 5% beta-mercaptoethanol, etc (See list of compatible reagents on BioRad cataloge) | Minimum incubation time 15minutes. Assay wavelength 650-750nm | |
| GENO TECHNOLOGY(#786-005) | Non-Interfering Protein AssayTM | Compatible with reducing agents(2ME, DTT), chelating agents EDTA , detergents (non-ionic, anionic, cationic, and zwitterionic) , amines (Tris), sugar, urea, ammonium sulfate, guanidine hydrochloride, guanidine thiocyanate, drugs, antibiotics, cobalt, and numerous other agents. | Quantitative precipitation of proteins while leaving interfering substances behind. | ||
| MOLECULAR PROBES (N-6666) | Nano Orange Protein Quantitation Kit (pdf) | 10 ng/mL - 10 µg/mL | Little protein-to-protein variability. Compatible with the presence of reducing agents and nucleic acids. | Mix and heat 10' 95ºC. Fluorescence emissions are measured directly | Unusually high concentrations of lipids in the sample can interfere. This interference can be eliminated by acetone precipitation of the protein, followed by delipidation with diethyl ether. |
| MOLECULAR PROBES (C-6667) | CBQCA Protein Quantitation Kit (pdf) | 10 ng/mL - 150 µg/mL | Functions well in the presence of lipids and detergents (to determine the protein content of lipoprotein samples or lipid–protein mixtures) | ||
| PIERCE (#23225) | BCA Protein Assay (pdf) | 0.5-20 µg/ml | Compatible with detergents solubilized proteins. Proteins on affinity supports. Chaotropic agents, sugars, DNA, protease inhibitors | 2ml reagent + 0.1ml sample Incubation 30' 37ºC. Read 562nm | Reducing agents (can be eliminated with TCA, see Protocol) |
| PIERCE (#23235) | Micro-BCA Protein Assay(pdf) | 0.5-20 µg/ml | Compatible with detergents solubilized proteins. Proteins on affinity supports. Chaotropic agents, sugars, DNA, protease inhibitors | 1ml reagent + 1ml sample Incubation 60' 60ºC. Read 562nm | Reducing agents (can be eliminated with TCA, see Protocol) |
| PIERCE (#23236) | Coomassie Plus Protein Assay | 1-1500 µg/m | For quick estimation where accuracy is not important. Reducing agents. Chaotropic and chelating agents, metals, protease inhibitors. DNA. | 3 ml reagent + 0.1ml sample Vortex and read 595nm | Detergents (sometimes you can normalize using same detergent concentration in blank and standarts) |
| PIERCE(#23200) SIGMA (#610-A) | Coomassie Protein Assay(pdf) BRADFORD | 1-1500 µg/ml | For quick estimation where accuracy is not important: great variability. Compatible with reducing agents. Chaotropic and chelating agents, metals, protease inhibitors. DNA. | 5 ml reagent + 0.1ml sample Vortex and read 595nm | Detergents (sometimes you can normalize using same detergent concentration in blank and standarts) |
| PIERCE (#23240) | Modified Lowry Protein Assay(pdf) | 1-1500 µg/ml | Accurate. Compatible with protease inhibitors. DNA. | 1 ml reagent + 0.2ml sample. Incubate 10' RT. Add 0.1ml Folin-Ciocalteu. Incubate 30' RT. Read 750nm | Reducing agents (can be eliminated with TCA, seeProtocol,pdf) and some detergents |
| PIERCE (#23255) | Fluoraldehyde Protein/Peptide Assay | 0.05-500 µg/ml | Compatible with reducing agents and some detergents. Chelating agents, metals and sugars | 2 ml reagent + 0.2ml sample. Mix and read fluorescence; excitation 330-390nm; emission 436-475nm. | Great variability. Cannot meassure in the presence of TRIS or Glycine buffer. |
| ROCHE (#1 767 283) (#1 767 003) | ESL Protein Assay (pdf) | 20-800 µg/ml. Detection limit 1µg/sample | Biuret-like reaction, detect peptide bonds. The assay is compatible with several detergents. Coul be used for determination of peptides and immobilized proteins | 7 minutes reaction. Read at 485nm. | |
| SIGMA (#690-1) | BIURET | 1-10 mg/ml | Very accurate and simple | Read 550nm | Glucose, Ammonium sulphate, Sulphydryl compounds, PO4 buffers |
| Folin-Ciocalteu reagent:SIGMA(#F9252) - MERCK | LOWRY | 10 µg/ml - 1 mg/ml | Very accurate | 1 ml reagent + 0.2ml sample. Incubate 10' RT. Add 0.1ml Folin-Ciocalteu. Incubate 30' RT. Read 750nm | Many detergents, reducing agents, EDTA, GuHCl, AmmSO4, >0.1M TRIS. Interfernce elimination withTCA or DOC-TCA |
| SIGMA(#P5656) Folin-Ciocalteu reagent:SIGMA(#F9252) - MERCK | LOWRY-PETERSON | 1-10 µg protein | Modified Lowry for membrane proteins. Compatible with detergents and with the presence of all kind of interferents that can be eliminated by DOC-TCA precipitation. | DOC-TCA precipitation of proteins before Lowry assay | |
| BUTTERFLY (coomassie staining into 3MM Whatman paper) | Very useful technique for proteins in all kind of solutions (like PAGE-SDS sample buffer) | Dry samples into 3MM Whatman paper.Treate with coomassie staining solution for ~30 min. Distain. Extracted ON with 3% SDS solution and read at 590 nm |
IlluminaProteinPrep可检测9,500种独特的人类蛋白质靶标，为蛋白质生物学提供全新洞察，推动疾病治疗新发现早期试用客户充分认可了其大规模检测能力、高度一致性以及简便的“从样本到洞察”......
调控基因组元件的高阶三维(3D)组织为基因调控提供了拓扑基础，但尚不清楚哺乳动物基因组中的多个调控元件如何在单个细胞内相互作用。2023年8月28日，北京大学汤富酬团队在NatureMethods（I......
多年来，出现了多种“大脑地图”，每一种都关注不同的大脑过程，从新陈代谢到认知功能。虽然这些地图很重要，但单独使用它们会限制研究人员从中得出的发现。现在，来自蒙特利尔神经学研究所的一个团队和其他研究人员......
蛋白质作为构成人体组织器官的支架和主要物质，在人体生命活动中起着重要作用。蛋白质的相互作用能产生许多效应，如形成特异底物作用通道、生成新的结合位点、失活、作用底物专一性和动力学变化等，细胞的代谢、信号......
蛋白质作为构成人体组织器官的支架和主要物质，在人体生命活动中起着重要作用。蛋白质的相互作用能产生许多效应，如形成特异底物作用通道、生成新的结合位点、失活、作用底物专一性和动力学变化等，细胞的代谢、信号......
2021年9月9日，无锡臻和生物科技有限公司（以下简称“臻和科技”）与美国VyantBio公司签署TissueofOrigin®（以下简称“TOO®”）全球权益和ZL转让协议，全资收购这款唯一获FDA......
2021年7月20日，JournalofCellularPhysiology及JournalofCellularBiochemistry同时撤回了中国学者49篇文章。从2019年开始，Journalo......
天然免疫应答是机体应对病原微生物入侵的第一道防线，在杀伤病原微生物、清除感染细胞和维持体内稳态等方面发挥关键作用。蛋白质翻译后修饰（proteinpost-translationalmodificat......
脂滴是一种具有中性脂质核心的膜性细胞器。与其他膜性细胞器的根本区别就是脂滴由单分子磷脂膜包被，而其他膜性细胞器由双分子磷脂膜与外界隔离。脂滴最早由列文虎克于1674年在牛奶里发现，是人类最早发现的膜性......
磷酸甘油酸突变酶1（PGAM1）通过其代谢活性以及与其他蛋白质（例如α平滑肌肌动蛋白（ACTA2））的相互作用，在癌症代谢和肿瘤进展中起关键作用。变构调节被认为是发现针对PGAM1的高选择性和有效抑制......