发布时间:2019-08-08 23:33 原文链接: NativeAcrylamideGels(35ml)

Native Acrylamide Gels (35 ml)


3.5%5%6%8%
30/0.8% acrylamide4.1 ml5.8 ml7 ml9.3 ml
10X TBE3.5 ml3.5 ml3.5 ml3.5 ml
H2027.4 ml25.7 ml24.5 ml22.2 ml

Degas 1-2 min

Add 0.25 ml 10% ammonium persulfate

Add 30 microliters TEMED

Pour Gel and wait 20-30 minutes before removing gel comb

Run at 180 volts in 1X TBE Buffer