NativeAcrylamideGels(35ml)
Native Acrylamide Gels (35 ml)3.5%5%6%8%30/0.8% acrylamide4.1 ml5.8 ml7 ml9.3 ml10X TBE3.5 ml3.5 ml3.5 ml3.5 mlH2027.4 ml25.7 ml24.5 ml22.2 mlDegas 1-2 minAdd 0.25 ml 10% ammonium persulfateAdd 30 microliters TEMEDPour Gel and wait 20-30 minutes before removing gel combRun at 180 volts in 1X TBE Buffer......阅读全文
Native Acrylamide Gels (35 ml)
Native Acrylamide Gels (35 ml)3.5%5%6%8%30/0.8% acrylamide4.1 ml5.8 ml7 ml9.3 ml10X TBE3.5 ml3.5 ml3.5 ml3.5 mlH2027.4 ml25.7 ml24.5 ml22.2 mlDegas 1-
Preparation of Agarose Gels for DNA separations
Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer, e.g. for an 0.8% gel, add
Cloning of small RNAs with 5’ phosphate and 3’ OH ends-2
3’ Adaptor Ligation and Purification Heat shock the RNA by putting at 90°C for 30 seconds. Snap cool on ice. Set up the 3’Adaptor ligation reac
非变性胶蛋白电泳
Section 2.1Nondenaturing Polyacrylamide Gel Electrophoresis of ProteinsJohn M. Walker1. IntroductionSDS-PAGE (Section 2.2) is probably the most commo
Lipoprotein Analysis Week 2: Electrophoresis
Lipoprotein Analysis Week 2: Electrophoresis IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification pr
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 130 mmMedium: 165 x 200 mmLarge: 165 x 260 mm5% Anal
Acrylamide Urea Gel (35 ml)
Acrylamide Urea Gel (35 ml)10%15%40/2% acrylamide10 ml13.1 ml10X TBE3.5 ml3.5 mlUrea15 g15 gH2010ml7.0 ml Microwave ~10 seconds and stir until dissolv
NuPAGE Gels
NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are inten
RNA Electrophoresis
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location
RNA电泳实验方法
Polyacrylamide Gel Electrophoresis (PAGE)for use withRibonuclease Protection Assay (RPA):1. Making the Gel: 5% Denaturing gel for Ribonuclease Protec