Direct introduction of DNA into plant protoplasts facilitates a rapid analysis of transient gene expression, as well as the generation of stably transformed transgenic plants. Transient gene expression assays performed after DNA transformation permit a comparative analysis of cis–actmg regulatory sequences and then function m transcriptional control of plant genes by signaling pathways mediating cellular responses to different environmental and hormonal stimuli (1). There are a number of methods for introducing DNA into plant protoplasts, but the most commonly used technique 1s the polyethylene glycol (PEG)–mediated DNA uptake. The PEG–mediated transformation is simple and efficient, allowing a simultaneous processing of many samples, and yields a transformed cell population with high survival and division rates (2). The method utihzes inexpensive supplies and equipments, and helps to overcome a hurdle of host range limitations of Agrobactenum–mediated transformation The PEG–mediated DNA transfer can be readily adapted to a wide range of plant species and tissue sources.
分析测试百科网讯2020年9月15日,2020年中国质谱学会质谱网络研讨会(2020CMSS)进行到第二天,由北京生命科学研究所董梦秋研究员、中国科学院生态环境研究中心汪海林研究员、同济大学田志新教授......
英国《自然》旗下新刊《自然·生物医学工程》近日在线发表的一篇论文,描述了一种由全新方法合成的不含抗原的糖尿病药物,不但能大幅延长药物在小鼠体内的作用时间,还消除了患者体内有预存抗体导致的药物副作用。在......
2013年12月2日消息,欧洲化学品管理局(ECHA)发布了一份为统一分类及标签(harmonisedclassificationandlabelling,CLH)的档案文件准备的指导草案的修订版,并......
应用PEG化干扰素成功实施的这一病例,具有一定的临床意义 其实,不单单在电视剧中,现实生活中也总会不时发生着一些活供体移植的感人故事。发生在日本的一例活体肝移植病例,在感人的成分之外,医学专......
摘要:简述了蛋白质PEG修饰的研究概况,涉及PEG化学修饰反应的类型和影响因素,PEG修饰蛋白的性质、应用及局限性。......