RetosBuffers&MediaBook

发布时间：2019-04-22 23:21 原文链接： RetosBuffers&MediaBook

08-15 Buffer


Compound	MW	Final concentration	200 ml
Glycine	75.07	0.1 M	1.5 g
1 M MgCl₂		10 mM	2 ml
KOH		to pH 9
H₂O			to 200 ml

A

Acetic Acid 1 M


Compound	MW	Final concentration	50 ml
Acetic Acid 98% (~17 M)		1 M	3 ml
H₂O			to 50 ml

Acrylamide/Bisacrylamide


Compound	MW	Final concentration	500 ml
Acrylamide	71.08	20 %	100 g
Bisacrylamide	154.17	0.8 %	4 g
H₂O			to 500 ml

Alkaline Lysis Buffer A


Compound	MW	Final concentration	500 ml
Glucose	180	50 mM	4.5 g
Tris	121	25 mM	1.5 g
EDTA 0.25 M		10 mM	20 ml
H₂O			to 450 ml
Adjust pH 8.0 with HCl
H₂O, Autoclave, store at 4°C			to 500 ml

Alkaline Lysis Solution 1


Compound	MW	Final concentration	100 ml
Lysozyme		0.2 mg/ml	0.02 g
Alkaline Lysis Buffer A			to 100 ml

Alkaline Lysis Solution 2


Compound	MW	Final concentration	50 ml
SDS 20 %		1 %	2.5 ml
NaOH 10 M		0.2 M	1 ml
H₂O			to 50 ml

Alkaline Lysis Solution 3


Compound	MW	Final concentration	50 ml
KAc	98	3 M	14.7 g
Acetic Acid 100 %		11.5 %	5.75 ml
H₂O			to 50 ml

Amido Black


Compound	MW	Final concentration	100 ml
Amidoblack	98	0.1 %	0.1 g
Acetic Acid 100 %		7.5 %	7.5 ml
Methanol		20 % (v/v)	20 ml
H₂O			to 100 ml

Aminoacid Concentration in Media


Constituent	Final mg/l	Stock	ml stock for 1 liter medium
Adenine sulfate	20	2 mg/ml	10
Uracil	20	2 mg/ml	10
L-tryptophan	20	2 mg/ml	10
L-histidine-HCl	20	2 mg/ml	10
L-arginine-HCl	20	2 mg/ml	10
L-methionine	20	2 mg/ml	10
L-tyrosine	30	3 mg/ml	10
L-leucine	30	3 mg/ml	10
L-isoleucine	30	3 mg/ml	10
L-lysine-HCl	30	3 mg/ml	10
L-phenylalanine	50	5 mg/ml	10
L-glutamic acid	100	10 mg/ml	10
L-aspartic acid	100	10 mg/ml	10
L-valine	150	15 mg/ml	10
L-threonine	200	20 mg/ml	10
L-serine	400	40 mg/ml	10

3-Aminophtalhydrazide 250 mM


Compound	MW	Final concentration	10 ml
3-Aminophtalhydrazide	177	250 mM	442 mg
H₂O			to 10 ml

Ammoniummolybdate 4.2 %


Compound	MW	Final concentration	50 ml
Ammoniummolybdate x 4H₂O	236	4.2 %	2.1 g
H₂O			to 30 ml
HCl fuming 37 % (~12 M)		4 M	16.7 ml
H₂O			to 50 ml

Ampicillin 20 mg/ml pH 8.0


Compound	MW	Final concentration	15 ml
Ampicillin	349.4	20 mg/ml	300 mg
H₂O			to 10 ml
Adjust pH 8.0 with NaOH
H₂O, Filter sterili 其他网友还关注过 Reto's Buffers & Media Book Reto's Buffers & Media Book2 Raft culture media (aka Green’s media) Yeast Media Antibiotic Concentration in Media Tissue Culture Media Cell Culture Media and Solutions Cell Culture Media and Solutions Bacterial Media Solutions and Stocks MEDIA FOR EMBRYO CULTURE AND MANIPULATION 更多与 RetosBuffers&MediaBook 相关的新闻分析测试百科网