RetosBuffers&MediaBook
08-15 BufferCompoundMWFinal concentration200 mlGlycine75.07 0.1 M1.5 g1 M MgCl2 10 mM2 mlKOHto pH 9H2Oto 200 mlAAcetic Acid 1 MCompoundMWFinal concentration50 mlAcetic Acid 98% (~17 M)1 M3 mlH2Oto 50 mlAcrylamide/BisacrylamideCompoundMWFinal concentration500 mlAcrylamide71.0820 %100 gBisacrylamide154.170.8 %4 gH2Oto 500 mlAlkaline Lysis Buffer ACompoundMWFinal concentration500 mlGlucose18050 mM4.5 gTris1212......阅读全文
Retos－Buffers－－Media－Book
08-15 BufferCompoundMWFinal concentration200 mlGlycine75.07 0.1 M1.5 g1 M MgCl2 10 mM2 mlKOHto pH 9H2Oto 200 mlAAcetic Acid 1 MCompoundMWFinal concent
Retos－Buffers－－Media－Book2
MgSO4 1 MCompoundMWFinal concentration15 mlMgSO4246.51 M3.70 gH2Oto 15 mlMRSCompoundMWFinal concentration1000mlTrypticase Peptone (BBL)1 %10 gBeef Ext
Raft－culture－media－(aka－Green’s－media)
Raft culture media (aka Green’s media)3 parts DMEM (including glutamine or glutamax)1 part Ham’s F125% FCSvarious supplements (not everybody uses the
Yeast－Media
YEPD (non-selection)-1% yeast extract-2% peptone-1.5% agar (if needed for plates)After autoclaving add glucose to 2% by adding 100 ml of 20% solution
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a
Antibiotic－Concentration－in－Media
Antibiotics should be added to lower than 60 C broth, or filter sterilized: See note. "C'' refers to chromosomal resistance: "P" plasmid based
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml fetal bovine serum, heat inactivated12.0 ml 200mM (100
Cell－Culture－Media－and－Solutions
Cell Culture Media and SolutionsDec. 18, 1990R. VeileAntimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml f
Bacterial－Media－Solutions－and－Stocks
3 agar (200 ml)Add 6 grams agar to 200 ml deionized water. Autoclave to sterilize.1.6 agar (200 ml)Add 3.2 grams agar to 200 ml deionized water.Autocl
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department)For oocyte maturation and routine culture of mouse emb
Yeast－Media,－Solutions－and－Stocks
Yeast Media:Note: Synthetic complete medium can be prepared by adding media supplements (see below).Medium using 6.7 g yeast nitrogen base without ami
细菌培养基
Preparation of LB Plate (Dr. Chastain)prepare LB plate with or without antibioticsBacterial Culture Media Recipes (WUGSC) M9 Plate Supplement (Gottsch
TISSUE－CULTURE－STOCK－SOLUTIONS－AND－MEDIA
MS MEDIUM FOR ARABIDOPSISTo 990 ml H2O add: Sucrose ........... 10.0 g MOPS .............. 0.5 g Agar .............. 8.0 g Adjust pH to 5.7
Embryonic－limb－bud－culture－in－media
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T
革兰氏阳性菌的电转化方案（英文）
Transformation of Gram-Positive Bacteriaan adaptation from Chang, D., Chassy, B., Saunders, J., Sowers, A. 1992.Guide to Electroporation and Electrofu
MEDIA－AND－SOLUTIONS－REQUIRED－FOR－ROUTINE－ES－CELL－CULTURE
Media UsedTo prepare 100 ml mediumDMEM80 mlFCS15 mlNon-essential amino acids (100x)1 mlPen/strep (5,000 1U/ml, 5000 ug/ml)1 mlL-Glutamine 200 mM1 mlNu
AA－－Metabolite－Quantitation－of－Media－PostAA－Labeling
1) Remove 2 500 µl aliquots of supernatant into scintillation vials, add scintillation fluid and count.2) Aliquot 1.6 mls of the remaining supernatant
Human－Embryonic－Stem－(ES)－Cell－Protocols——Media－and－Reagents
Serum Free Media for human ES cells on MEFs: can last for 7-10 daysFinal ConcentrationAmount for 250ml Stock solution80% DMEM-F12200ml20% KO Serum Rep
Tissue－Culture－Methods3
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach student should m
Cell－Sorting－By－FACS
Currently, the Moflo instrument (Dakocytomation) is used to sort cells at the AECOM FACS facility.Sorting is performed by the person in charge at the
GMP级干细胞细胞冻存液－Cryopreservation－Media
产品描述： STEM-CELLBANKER ®是GMP下生产的ES细胞和iPS细胞专用冻存液 产品特点是化学成分清晰，无动物源成分，专门针对ES细胞和iPS细胞特点设计的细胞冻存液。STEM-CELLBANKER ®是完全无血清和动物源性成分的，所有成分完全满足欧洲药典或美国药典的要求，是
Protocol－for－Cell－Fusion
Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, the
Screening－Bacterial－Colonies－Using－Xgal－and－IPTG:－αComplementation
实验概要 α-complementation occurs when two inactive fragments of E. coli β-galactosidase associate to form a functional enzyme. Many plasmid
Collagen－gel－containing－3T3－fibroblasts－dermal－equivalent－for－raft－culture
Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)Ingredients for 6 x collagen matrices in a 6-well plate;Roughly 3x106 J2-3
Expression－Protocol－in－M9－Minimal－Media－via－T7－Promoter
The following protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 min
Expression－Protocol－in－M9－Minimal－Media－via－T7－Promoter
Expression Protocol in M9 Minimal Media via T7 PromoterThe following protocol has been used successfully to 15N or 13C/15N label our proteins using ou
Preparation－of－Lactobacillus－Competent－Cells
OverviewInstructions on how to prepare Lactobacillus plantarum competent cells before electrotransformation.MaterialsMRS mediaCulture of L. plantarum
常用试剂配制－5
Sodium citrate (MW 294.10)0.09 MDissolve 2.65 grams of sodium citrate to a final concentration of 100 ml with water.Sodium citrate/formaldehyde (for s
CellTrace™－CFSE－Cell－Proliferation－Kit
实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use form. The
基本实验技术
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are required by