厉害了！华人学者今日连发4篇Cell
顶尖学术期刊《细胞》在线刊登了6篇最新的研究。我们很高兴地看到，其中4篇研究来自华人学者的研究团队。其中，更有两项研究的科研人员为“全华班”。 1 Gene-Wei Li教授课题组 Evolutionary Convergence of Pathway-Specific Enzyme Expression Stoichiometry 在基因调控中，由通路诱导信号带来的蛋白质共表达是基础中的基础。但人们尚未明确共表达的蛋白质相对含量是否也受到了精确的调控。在本研究中，来自MIT的Gene-Wei Li教授团队进行了一项大规模的实验，对不同细菌中的21条信号通路与67-224个操纵子进行了化学计量上的分析。利用一种特殊的RNA测序技术（Rend-seq），研究人员们以单核苷酸的分辨率，发现在大量编码细菌保守通路的基因簇里，其内部的启动子和终止子会出现重排，带来大量的转录水平多样性。令人震惊的是，演化巧妙地通过转录后调控的......阅读全文
uCalpain－and－friends－in－Cell－spread
The mammalian calpain gene family curently contains 13 distinct large subunit products most of which complex with one of two smaller 30kDa subunits. (
B－Cell－Receptor－Complex
B cells produce immunoglobulins (Ig, antibodies) that specifically bind antigen molecules. B cells first produce a membrane-bound form of immunoglobul
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml fetal bovine serum, heat inactivated12.0 ml 200mM (100
mCalpain－and－friends－in－Cell－motility
The mammalian calpain gene family currently contains 13 distinct large subunit products most of which complex with one of two smaller 30kDa subunits.
MTT－Cell－Proliferation－Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondri
Subculture－of－Adherent－Cell－Lines
AimAdherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the
T－and－B－cell－Isolation
Reagents Heparin - 1000 U/ml Ficoll-Hypaque PBS RPMI-1640 supplemented with 10 mM glutamine and 15% FBS AET (0.14M) Dissolve 1.967 g AET in 35 ml di-H
Bystander－B－Cell－Activation
Expression of Fas-Ligand by T cells can induce apoptosis through interaction with Fas on the target cell. A target of Fas-mediated apoptosis is the B
immunofluorescence－of－general－cell－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of general cell by Peprotech.实验步骤Please refer to the antibody Product Information S
Resuscitation－of－Frozen－Cell－Lines
AimMany cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use them the cells must be thawed and put into
T－cell－Activation－Protocol
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
AlamarBlue®－Cell－Viability－Assay
实验概要Assess cell viability. 实验原理Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activ
Live－Cell－Imaging－of－Yeast
Live Cell Imaging of YeastDaniel R. Rines, Dominik Thomann, Jonas F. Dorn, Paul Goodwin and Peter K. SorgerINTRODUCTIONThe development of cloning vect
SDS－Whole－Cell－Extracts
-Use sterile technique and sterile solutions in steps 1 to 3.-1. Using a saturated starter culture, inoculate 25 to 30 ml of appropriate media in a 12
Cell－Culture－Media－and－Solutions
Cell Culture Media and SolutionsDec. 18, 1990R. VeileAntimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml f
Cell－Suspension－Culture－of－Arabidopsis
实验概要Cell Suspension Culture of Arabidopsis 主要试剂10% (v/v) Household BleachCallus Induction Medium Gamborg's B5 Basal Medium 0.5 g/liter M
Cell－and－tissue－lysis－hub
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
Cyclins－and－Cell－Cycle－Regulation
The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted f
Cell－counting－with－an－hemacytometer.
Accuracy of manual counts with an hemacytometer depend on:accurate mixing of the sample (no bubbles!)number of chambers countednumber of cells counted
Cell－Disruption－and－Subcellular－Fractionation
Nitrogen Bomb1) Harvest cells and wash 2 times with PBS.2) Resuspend final pellet in relaxation buffer. (20 ml/1X109 cells)3) Pressurize with N2 for 2
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
In－Vitro－T－Cell－Activation
In Vitro T Cell ActivationIntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR).
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
B－Cell－Survival－Pathway
Physical interactions between intergrin alpha4beta1 heterodimer expressed on B cells and counter receptors on stroma cells are key mediators of the su
Cell－Clonogenic－Survival－Assay
DescriptionAllows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure1. Grow
Cell－Stem－Cell：细胞命运变化中染色质开关规律
信息时代是计算机语言的二进制码（0-1）驱动的，0与1二进制演绎出丰富多彩的虚拟世界，包括热门的人工智能AI。那么，生命科学是否也存在类似的0-1二进制规律的密码？ 中国科学院广州生物医药与健康研究院裴端卿、陈捷凯课题组通过对干细胞命运诱导过程的研究，发现细胞命运转换也遵循一个二进制规律。科学
张素春教授Cell－Stem－Cell：当细胞疗法遇上CRISPR
来自威斯康星大学麦迪逊分校的神经科学家们将一种遗传开关插入到了神经细胞中，使得患者可以通过服用不影响任何其他细胞的设计药物来改变它们的活性。正在研究的细胞是可以生成神经递质多巴胺的神经元，其发生缺陷是广泛运动障碍帕金森病的罪魁祸首。 多巴胺是对协调运动至关重要的一种大脑化学物质。帕金森病标准疗
中山大学Cell－Stem－cell发布表观遗传重要成果
来自中山大学生命科学学院、Baylor医学院的研究人员证实，在DNA低甲基化时Daxx/Atrx复合物通过促进H3K9三甲基化（H3K9me3）保护了串联重复元件（Tandem Repetitive Elements）。这一重要的研究发现发布在9月3日的《细胞干细胞》（Cell stem Cel
李家洋应邀在PLANT－CELL撰写Reflections－on－Plant－Cell－Classics文章
The Plant Cell是植物领域的著名学术期刊，对植物学的发展起到了重要的引领作用。为庆祝创刊30周年，The Plant Cell杂志社邀请部分编委会成员及其他科学家对发表在该杂志的重要研究工作进行评述，以期增进对植物科学过去30年中重要研究进展的认识，激发对未知领域的探索。 李家洋研
继Science－Cell－Res后－何祖华团队登Mol－Cell
2019年4月9日，国际著名学术期刊Molecular Cell在线发表了中国科学院分子植物科学卓越创新中心/植物生理生态研究所何祖华研究组完成的关于水稻广谱抗病的最新研究成果 “RRM Transcription Factors Interact with NLRs and Regulate