AntigenRetrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortunately, this process can result in the cross-linking of proteins which can mask binding sites on the antigen of interest. In order to unmask, or retrieve, these cross-linked sites, scientists can utilize a variety of procedures known colle......阅读全文
Lck－and－Fyn－tyrosine－kinases－in－initiation－of－TCR－Activation
T cell activation is initiated by recognition of antigen by the T cell receptor (TCR) in the context of Class II MHC on an antigen-presenting cell. Th
BCR－Signaling－Pathway
Significant progress has been made towards delineation of the intrinsic molecular processes that regulate B lymphocyte immune function. Recent observa
ELISA－Inhibition－Assay
ELISA Inhibition AssaySensitize a 96-well microtiter plate with purified antigen.Prepare a solution of the purified antigen of interest in phosphate b
Indirect－ELISA
实验概要 In the indirect ELISA, the enzyme-antibody conjugate uses an antibody against the type of antibody that is used to detect the antigen, kind of
Polyclonal－Antibody－Production
实验概要Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.实验步骤Rabbit Immunization Initial
与细胞结构及微环境信号通路相关因子介绍EPCAM
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
细胞骨架调节及运输信号通路相关概念EPCAM
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
细胞结构及微环境信号通路相关EPCAM
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
与细胞骨架调节及运输信号通路相关因子介绍EPCAM
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
FAF1基因突变与药物因子介绍
Fas配体（TNFSF6）与Fas抗原（TNFRSF6）的相互作用介导许多器官系统中的程序性细胞死亡，也称为凋亡该基因编码的蛋白与FAS抗原结合，通过FAS抗原启动细胞凋亡或增强细胞凋亡由该基因编码的蛋白启动凋亡需要泛素样结构域，而不是fas结合结构域。[由RefSeq提供，2008年7月]Inte
EPCAM基因编码功能及结构描述
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
实体肿瘤检测EPCAM基因介绍
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
遗传风险基因信号通路相关因子EPCAM
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
EPCAM基因突变与药物因子介绍
该基因编码癌相关抗原，是一个家族的成员，至少包含两种I型膜蛋白。这种抗原在大多数正常上皮细胞和胃肠道癌上表达，并作为一种同型钙依赖性细胞粘附分子发挥作用。该抗原正被用作人类癌免疫治疗的靶点。该基因突变导致先天性丛生性肠病。This gene encodes a carcinoma-associate
FAF1基因编码功能及结构描述
Fas配体（TNFSF6）与Fas抗原（TNFRSF6）的相互作用介导许多器官系统中的程序性细胞死亡，也称为凋亡该基因编码的蛋白与FAS抗原结合，通过FAS抗原启动细胞凋亡或增强细胞凋亡由该基因编码的蛋白启动凋亡需要泛素样结构域，而不是fas结合结构域。[由RefSeq提供，2008年7月]Inte
Cytokine－Network
Several different cell types coordinate their efforts as part of the immune system, including B cells, T cells, macrophages, neutrophils, basophils an
Bystander－B－Cell－Activation
Expression of Fas-Ligand by T cells can induce apoptosis through interaction with Fas on the target cell. A target of Fas-mediated apoptosis is the B
肿瘤特异性抗原的概念
肿瘤特异性抗原（tumor specific antigen）指仅表达于某种肿瘤细胞表面而不存在于正常细胞上的新抗原，故又称独特肿瘤抗原（unique tumor antigen）。
ELISPOT－(Enzymelinked－ImmunoSPOT)－实验方法步骤1
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
Basic－ELISA－Protocol
实验概要 There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common typ
Dendritic－cells－in－regulating－TH1－and－TH2－Development
While T cells and B cells carry out the actions of antigen-specific immune responses, antigen-presenting cells called dendritic cells are required for
B－Lymphocyte－Cell－Surface－Molecules
B cells must communicate with their environment to participate in the immune system as the source of antigen-specific immunoglobulins. Cell surface pr
Blocking－With－Immunizing－Peptide－(BL)－Protocol
实验概要Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodi
Dynabeads®－Antibody－Coupling－Kit
实验概要Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodi
Th1/Th2－Differentiation
Helper T cells are found in two distinct cell types, Th1 and Th2, distinguished by the cytokines they produce and respond to and the immune responses
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
免疫学嗜异性凝集吸收试验介绍
人、动物、植物、微生物间存在一种嗜异性抗原(Forssman antigen，F.antigen)，可激发机体产生与种属特异性无关的嗜异性抗体(嗜异性凝集素)(Forssman antibody，F.antibody)，除与相应抗原起反应外，还能与其他动物的红细胞或组织浸液等发生反应。检测患者血清中
免疫资料——免疫学常用简称介绍
1：抗体查询常用名词Reactivity （物种与抗体反应）：Species with which the antibody reactsHost Species （宿主）：Host in which the antibody was generatedApplications（应用） ：Speci
前列腺抗原－总前列腺和游离前列腺比值的意义
1 前列腺抗原应该指的是前列腺特异性抗原（prostate specific antigen，PSA），它是一种与前列腺癌相关的抗原，生理条件下主要由前列腺导管上皮细胞合成，分泌人精浆，微量进人血液循环。2 总前列腺应该指的是总前列腺特异性抗原（total prostate specific ant
Agglutination－Assays
Agglutination Assays REFERENCE: Lanyi, B., and T. Bergan. Methods in Microbiology, Vol 10: 93-168. BACTERIAL AGGLUTINATION: Bacterial agglutinat