TheOP9DL1System:GenerationofTLymphocytesfromEmbryonic2
Freezing ESCs 25. Passage the ESCs as described in Step 22. Resuspend the cells in 3 mL of freezing medium for OP9 cells. 26. Aliquot 1 mL of cell suspension per cryovial (~3-6 x 105 cells/cryovial). 27. Freeze the cells at -80°C, and then transfer them to liquid nitrogen for storage. Establishing ESC/OP9-DL1 Cell Co-culturesDay 0: Initiation of Co-culture S......阅读全文
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collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOHput in boiling H2 O for 30 sec (optimum may n
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