DorsalRootGanglionNeuronCulturefromAdultRats
DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. ProcedurePreparing Culture Surface- Collagen coat cover slips (if using) or cell culture dishes: evenly spread 2-3 drops of rat-tail collagen onto culture surface and allow to dry. May store overnight in cell culture hood or up to 7 days at 37C.Removing DRG- Euthanize animal- Remove spinal column from animal.&......阅读全文
Acetobacter－Xylinum－Culture
OverviewGeneral guidelines on how to grow up a culture of Acetobacter xylinum, ATCC strain 53582 .Preparation of Acetobacter MediaTo prepare ∼500 ml o
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a
Culture－of－Endometrial－Explants
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and ProstaglandinsP. J. Hansen and J.
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（二）
Materials and MethodsShort-term cell growth evaluationLonza™ Poietics™ human mesenchymal stem cells (hMSC-BM, PT-2501, Lonza) derived from normal ad
PCR－from－Tissue
collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOHput in boiling H2 O for 30 sec (optimum may n
PCR－from－Tissue
collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOHput in boiling H2O for 30 sec (optimum may ne
PCR－from－Tissue
1.collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH 2.put in boiling H2O for 30 sec (optimum
背根神经节的定义
中文名称背根神经节英文名称dorsal root ganglia定 义来源于神经嵴的脊神经节内的感觉神经母细胞发出轴突，呈束状，左右对称地从神经管的背部进入，此时的脊神经节即改称为背根神经节。应用学科遗传学（一级学科），发育遗传学（二级学科）
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
Nature：胰腺癌的神经黑客行动！秘癌细胞如何“策反”神经元加速扩散
在与癌症的博弈中，胰腺导管腺癌（PDAC）始终是难以攻克的堡垒，其五年生存率不足10%，素有“癌王”之称。传统研究聚焦于基因突变与免疫逃逸，却忽视了肿瘤微环境中一个隐秘的“共谋者”——周围神经系统。2月17日《Nature》杂志发布的一项突破性研究“Characterization of singl
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
活细胞成像在中枢神经系统（CNS）疾病和紊乱研...（三）
Huvec细胞： Huvec细胞（固定），用SiR-actin染色，共聚焦显微镜成像。 大鼠海马神经元： 用SiR-actin染色培养大鼠海马神经元的STED图像。肌动蛋白环（条纹）周期性为180nm。 MCF10A Cells
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Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
CO2恒温摇床解决人胚肾－293－(HEK293)－细胞结团问题（二）
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from confluent T-flasks or from suspension cultures at h
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra
Isolate－DNA－from－NRBC－Blood－from－Buccal－Swab－collected－with－filter－paper
实验概要DNA isolation from fish or avian blood sample can be difficult because it contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is de
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells3
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
Neuron：用光操纵记忆？
最近，加州大学戴维斯分校神经科学中心和心理学系的研究人员，利用光消除了小鼠脑中的特定记忆，并证明了一个关于“大脑不同部分如何共同工作来检索情景记忆”的基本理论。相关研究结果发表在最近的《Neuron》杂志。 光遗传学（Optogenetics），是斯坦福大学Karl Diesseroth首创的
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml fetal bovine serum, heat inactivated12.0 ml 200mM (100
Cell－Culture－Media－and－Solutions
Cell Culture Media and SolutionsDec. 18, 1990R. VeileAntimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml f
Stock－solutions－for－tissue－culture
The kitchen makes Tris, TD, Tryp/TD, PBS, and VE.Tris is a fairly complex, Tris-buffered physiological saline solution. It is used to wash cells and i
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department)For oocyte maturation and routine culture of mouse emb
In－vitro－culture－of－embryonic－lungs
In vitro culture of embryonic lungsfrom Hogan LabIsolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fetal bovine serumpetri
Preparing－Overnight－Bacterial－Culture
Materials:Sterile LB medium (Luria-Bertani Medium) with or without antibiotic:water 500 mlbacto-tryptone 10 gbacto-yeast extracts 5 gsodium chloride 1
Cell－Suspension－Culture－of－Arabidopsis
实验概要Cell Suspension Culture of Arabidopsis 主要试剂10% (v/v) Household BleachCallus Induction Medium Gamborg's B5 Basal Medium 0.5 g/liter M
stem－cell－culture－protocol
实验概要stem cell culture protocol主要试剂cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
ES－Cell－Culture－and－Manipulation
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
Immature－Embryo－Rescue－and－Culture
Embryo culture techniques have many significant applications in plant breeding, as well as basic studies in physiology and biochemistry. Immature
做科学－勇敢些
前段时间看到饶毅老师发的 “这是我们目前主流状况：冒险第一不行，第二很多”，我感觉很有意思。这句话看上去是在“骂人”，其实充满了赞扬，也包含着更高的期许。当然，在讨论前，我要把主题特异到“生物医学基础研究”这个方向，这个是我大体能懂的专业，也是一个非常大的且足够重要的领域。 先说“充满了赞扬”