NO2dependentIL12PathwayinNKcells
Macrophages and NK cells help provide innate immunity against infection by intracellular parasites and communicate with each other to regulate this process. When stimulated, macrophages secrete the cytokine IL-12 that is essential for activation of the cytotoxic activity of natural killer (NK) cells. IL-12 stimulates this NK cell response through activation of a JAK/STAT signaling pathway. Binding of IL-12 to its rec......阅读全文
Lectin－Induced－Complement－Pathway
The complement cascade of proteolytic factors involved in cellular lysis can be initiated by several different factors, including antibody-dependent a
B－Cell－Survival－Pathway
Physical interactions between intergrin alpha4beta1 heterodimer expressed on B cells and counter receptors on stroma cells are key mediators of the su
Hop－Pathway－in－Cardiac－Development
Homeodomain transcription factors comprise a large family of DNA binding factors that regulate transcription and development. Many homeodomain genes a
Growth－Hormone－Signaling－Pathway
Growth hormone plays a major role in regulating growth during childhood and adolescence and also regulates metabolism. Defects in growth hormone signa
Nitric－Oxide－Signaling－Pathway
Glutamatergic-mediated nitric oxide (NO) production occurs via the N-methyl-D-aspartic acid (NMDA) postsynaptic density protein 95 (PSD95)-neuronal ni
过继免疫治疗法的主要特点和原理
是取对肿瘤有免疫力的具有抗肿瘤活性的细胞输给患者，或取患者自身的免疫细胞在体外活化、增殖后再转输入患者体内，使其在患者体内发挥抗肿瘤作用。过继免疫治疗的效应细胞具有异质性，如CTL、NK细胞、巨噬细胞、淋巴因子激活的杀伤细胞（lymphokine-activated killer cells, LA
免疫细胞表面抗原分子CD家族对照表(CD1CD247)2
CD1cPositive staining (normal): subset of normal peripheral B cellsPositive staining (disease): myeloid leukemias and some B cell malignancies Negativ
Nat－Immunol：CIS——NK细胞抗肿瘤的新型免疫检查靶点
CD8效应细胞的功能受到多种调节性蛋白的影响。对这些叫做“检查点（checkpoint）”的蛋白质的鉴定以及特异性的阻断能够有效治疗癌症。例如，针对CD8 T细胞的抑制性受体“PD-1”的抗体能够有效阻断其与相应配体的结合，进而增强抗肿瘤CD8 T细胞免疫反应。然而，尽管针对CD8 T细胞抗肿瘤
Isolation－of－papillary－cells
实验概要This protocols provides a general protocol for isolation of papillary cells.实验步骤Isolation of renal papillary cells1. For isolation of papillary c
Electroporation－of－ES－cells
Cells are routinely passaged two days prior to electroporating. Usually one 10 cm plate at approximately 80% confluency will provide enough cells for
Subculturing－Adherent－Cells
实验概要The following protocol describes a general procedure for subculturing adherent mammalian cells in culture.主要试剂1. Complete growth medium, pre-warme
Immunofluorescence－Labeling－of－Cells
实验概要Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a very ver
Isolation－of－papillary－cells
Isolation of renal papillary cells1. For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Transfecting－Suspension－Cells
实验概要将转移基因整合到细胞染色体DNA上,形成稳定表达转移基因的细胞系。 实验原理 细胞转染技术是目前广泛应用于病毒基因结构与功能以及基因调控等的研究。细胞转染可分为短暂转染和稳定(或永久) 转染两种。在短暂转染中,被转染基因并不整合至细胞染色体中,因而不能随细胞分裂而传代。转入病毒基因的转
Collection－of－Peritoneal－Cells
Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important.Prepare a Pasteur pip
Collection－of－Peritoneal－Cells
Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important.Prepare a Pasteur pip
Freezing－and－Thawing－cells
Freezing and Thawing cellsFreezingIt is best to freeze cells that are growing rapidly. With adherent cells, it is easiest to set up 100 mm dishes, giv
Preparing－cells－and...
实验概要The method provides a protocol and tips for BrdU staining in tissue sections.Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic
Fluorescent－Staining－of－Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip.2. PB
KARYOTYPING－ES－CELLS
An actively growing culture of cells is required, i e 2 - 3 d ES cell culture. The total number of cells needs to be between 106 - 107 cells.N B Read
Cytokine－Network
Several different cell types coordinate their efforts as part of the immune system, including B cells, T cells, macrophages, neutrophils, basophils an
肝脏肿瘤如何逃脱自然杀伤细胞的“捕杀”？
NK细胞：正常细胞的“卫兵” 在人体中，存在着众多不同类型的细胞，自然杀伤细胞（Natural killer cells， NK cells）就是其中非常“奇妙”的一种。NK细胞是一种效应性免疫细胞，在机体抗肿瘤过程中发挥重要作用。它可以直接识别和攻击肿瘤细胞，还能间接通过分泌活性因子促进机体
临床化学检查方法介绍NK细胞活性测定（NK）介绍
NK细胞活性测定（NK）介绍： 自然杀伤(NK)细胞是一群异质性多功能的免疫细胞，是与特异性免疫应答无关的自然毒杀伤细胞。它对体内多种细胞，特别是T、B淋巴细胞有调节作用。它所介导的裂解细胞作用不受主要组织相溶性复合体的限制。自然杀伤细胞不仅对癌细胞，对病毒、胞内寄生菌和老化变异细胞也具有极强的清
科学家开发出新一代高效、低毒的肿瘤限制性IL15前体药物
8月10日，Cell Research在线发表了中国科学院生物物理研究所研究员彭华与美国德克萨斯大学西南医学中心教授傅阳心合作完成的研究论文Tumor-conditional IL-15 pro-cytokine reactivates anti-tumor immunity with limi
Cell－Research：新一代高效、低毒的肿瘤限制性IL15前体药物
8月10日，Cell Research在线发表了中国科学院生物物理研究所研究员彭华与美国德克萨斯大学西南医学中心教授傅阳心合作完成的研究论文Tumor-conditional IL-15 pro-cytokine reactivates anti-tumor immunity with limi
小鼠IL12p70－ELISA试剂盒检测前准备工作
1、请提前20分钟从冰箱中取出试剂盒，平衡至室温。2、将浓缩洗涤液用双蒸水稀释(1:25)。未用完的放回4℃。从冰箱中取出的浓缩洗涤液可能有结 晶，属于正常现象，可用40℃水浴微加热使结晶完全溶解后再配制洗涤液(加热温度不要超 过50℃，使用时洗涤液应为室温)。当日使用。3、标准品(冻干品)：每瓶标
两大小鼠模型在免疫治疗领域作为先行军的应用（二）
肿瘤免疫抗体药物研究中的应用 免疫检查点抗体药物能够通过调节免疫系统进行肿瘤治疗，免疫系统人源化小鼠能够提供人体免疫细胞，通过肿瘤移植可以验证和评价单克隆抗体、双特异性抗体及联合用药的药效。PF-05082566（Utomilumab, Pfizer）是一种全人源IgG2，可与人4-1
常见细胞因子开专场啦！
细胞因子检测试剂盒种类比较多，如白介素、选择素、集落刺激因子，肿瘤坏死因子，干扰素，转化生长因子，趋化因子，细胞因子受体，粘附分子，生长因子，凋亡因子等等！今天的文章中，上海恒远将为大家详细的介绍几种常见的细胞因子！ 一、白介素1、IL-2（CIK：24h后1000u /ml添加）（1）诱导细
白介素2对NK细胞的作用
IL-2可促进NK细胞的增殖，维持NK细胞长期生长。肿瘤病人经IL-2治疗后，血中NK细胞数量明显增加。IL-2在体内、外都能增强NK细胞活性。在体外，IL-2于短时间内就可使NK细胞活性增强。肿瘤病人经IL-2治疗后NK细胞活性变明显增强，且有累积效应，即随着IL-2剂量的增加和疗程的延长，NK细
抗炎因子IL10的功能与作用
白细胞介素10 (IL-10)，也被称为人类细胞因子合成抑制因子(CSIF)，是一种抗炎细胞因子。在人类中，白细胞介素10是由IL10基因编码的。IL-10信号通过两个IL10同源二聚体与一个由两个IL-10受体-1和两个IL-10受体-2蛋白组成的异源四聚体受体复合物传递。下游通过JAK1和T