TetrahymenaFixationforTransmissionElectronMicroscopy
Tetrahymena Fixation for Transmission Electron MicroscopyPellet Tetrahymena cells in a clinical centrifuge.OPTIONAL: Suspend cells in HNMK (50 mM HEPES, pH 6.9, 36 mM NaCl, 0.1 mM Mg acetate, 1 mM KCl ) for 10-20 min at room temperture. This will remove precipitates present in the proteose peptone and provide cleaner surfaces if cells are processed for SEM.Pour off most of the medium and suspend cells in a slurr......阅读全文
Immunofluorescence－Labeling－of－Cells
实验概要Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a very ver
胚胎和成年斑马鱼眼情的组织学准备
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
表界面科学最新文献KSV－NIMA
1. Name：Tuning structure and properties of ultra-low cross-linked temperature-sensitive microgels at interfaces via the adsorption pathwaysAuth
电子探针仪与扫描电镜有何异同
透射电子显微镜 (transmission electron microscopy﹐简写为TEM)。 构造原理 ： 电子显微镜的构造原理与光学显微镜相似﹐主要由照明系统和成像系统构成(图1 光学显微镜与电子显微镜的对比 )。照明系统包括电子枪和聚光镜。钨丝在真空中加热并在电场的作用下发射出电
ICBR－Flow－Cytometry－Core－Laboratory－Paraformaldehyde－Fixation－of－Cells
Background This fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light s
TEM－Visualization－of－Microtubules
LEVEL II Materials Coated grid for TEM 0.1 M ammonium acetate 5% ethanol saturated uranyl acetate Transmission electron microscope
材料形貌分析
相貌分析的主要内容是分析材料的几何形貌，材料的颗粒度，及颗粒度的分布以及形貌微区的成份和物相结构等方面。形貌分析方法主要有：光学显微镜(Opticalmicroscopy,OM)、扫描电子显微镜(Scanningelectron microscopy, SEM)、透射电子显微镜(Transmis
光合电子传递－（photosynthetic－electron－transport）
光合作用中，受光激发推动的电子从 H2 O到辅酶Ⅱ（ NADP ）的传递过程。光合色素吸收光能后，把能量聚集到反应中心——一种特殊状态的叶绿素 a分子，引起电荷分离和光化学反应。一方面将水氧化，放出氧气；另一方面把电子传递给辅酶Ⅱ（ NADP ），将它还原成 NADPH，其间经过一系列中间（电
EBSD分析（electron－backscatter－diffraction）是指
EBSD即电子背散射衍射。EBSD的原理始于20世纪50年代，技术问世于80年代。EBSD是扫描电子显微镜（SEM）的一个标准分析附件，但大大拓宽了扫描电子显微镜进行微观分析的功能。它可以与SEM的其他功能（包括EDS等配件）结合起来，原位成像、成分分析、大样品分析、粗糙表面成像等，克服了传统分析方
A－semipermanent－mounting－medium－for－immunofluorescence－microscopy
A semi-permanent mounting medium for immunofluorescence microscopy Materials 6gm glycerol 2.4gm mowiol 6ml distilled water 12ml Tris buf
第三次生物成像论坛在生物物理所召开
4月8日上午，来自孙飞、朱平、苗龙、徐涛研究组的研究人员和生物成像中心的高级技术人员，在生物物理所召开了第三次生物成像论坛。 会议首先回顾了生物成像中心成功建立300KV Titan Ciros 超高分辨率电镜后所取得的成绩，同时结合近期举办的能量过滤以及STEM培训活动，本
补体结合反应(complement－fixation－test)技术概况
可溶性抗原，如蛋白质、多糖、类脂质和病毒等，与相应抗体结合后，抗原抗体复合物可以结合补体，但这一反应肉眼不能察觉，如再加入红细胞和溶血素，即可根据是否出现溶血反应来判定反应系统中是否存在相应的抗原或抗体。这个反应就是补体结合反应。 补体结合反应是一种古老的血清学技术，Bordet和Gengou在19
如何选择电镜：不妨看看这75篇重要文献
这是一篇有关电子显微镜的综述，是根据75篇发表使用实验的文章归纳的。可以帮助读者找到最适合的电子显微镜。日立高新Hitachi High Technologies America为研究碳酸酐酶可通过spidroin蛋白末端功能域促进蜘蛛丝的形成，采用Hitachi的H7100 electr
我国揭示可指导材料精准制备的材料取向连接生长机制
在国家自然科学基金项目（项目编号：51420105003、11327901、11525415等）等资助下，孙立涛教授团队与国际项目合作单位美国伯克利劳伦斯国家实验室的郑海梅团队在材料取向连接生长机理方面取得突破。研究成果以“In-situ Liquid Cell Transmission Ele
Immunofluorescence－...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Use－of－SemiThin－Cryosections－for－Light－Microscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by secti
组织学——微波技术
Microwave Processing Techniques for Microscopy (Energy Beam Sciences, Inc.) Introduction to microwave technique and detailed protocol for its appli
《Nature》冉冉升起的新星之中国学者葛炳辉——电子显微镜
创造性思维驱动科学发展，起决定作用的永远是人，最新一期（9月19日）Nature杂志中的“Nature Index 2018 Rising Stars”介绍了在自然科学领域崭露头角的一群研究人员，他们也许正在定义着未来的科学研究。这11位职业生涯早期或中期的科学家所在的领域从削减太阳能电力的成本到降
线粒体荧光探针大全：TMRM，Mitotracker，JC1（1）
线粒体荧光探针信息大全 （Probes for Mitochondria）包括各种常用探针，如JC-1，JC-9，TMRM，TMRE等Mitochondria are found in eukaryotic cells, where they make up as much as 10% of th
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（五）
Single crystal sapphire is not a typical optical material, with a standard strength not much lower than the steel described above, since the av
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（六）
windows were confused by the simultaneous deflection of the windows and the O-ring before the window came in contact with the flange. The recesse
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（七）
The failure data is shown for the 12.5 mm diameter disks is shown in Fig. 12. The average failure pressure for the standard disks is 12 MPa, wh
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（九）
3) 50 mm Disks. Four 50 mm diameter, 2 mm thick disks were specially polished by Meller Optics to try to obtain strengthening disks. A previous p
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（八）
Strength Prediction. Large deflection theory solves for the deflection of a disk as a function of loading and boundary condition. Stress is a deri
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（三）
Sapphire Window Development and Testing.The effort of this work was devoted to fabricating ultrathin strengthened sapphire disks and to accurately
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十一）
Fixture Testing. Fixture testing consisted of vacuum, heating, pressure, and microwave testing.Pressure Testing. Initial pressure testing of thin sa
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十三）
The diameter uptaper from 38.3 mm to 63.5 mm was then fabricated to match the diameter of the resonant ring and windows to be tested. A square r
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（四）
Membrane effects describe the case where the disk stretches rather than bends. Figure 5 shows the differences in the deflection and stress for the
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十二）
The input port is connected to the high power gyrotron source and the output port is connected to a dummy load to absorb any power not coupled i
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十）
through a boundary conductance, h, on faces at z = +/- L.is controlled by the parameter hL/k, the Biot Number, where k is the thermal conductivity