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apipelinefortheidentificationofintactNglycopeptides(七)

Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Recently reported software tools used HCD- or CID-MS/MS to identify glycopeptides, especially the glycan moiety of glycopeptides, but few of them integrated these two fragmentations to further improve the glycopeptide identification5,20. GlycoFragWork combined HCDan......阅读全文

a pipeline for the identification of intact N-glycopeptides(四)

Interpretation of MS3 data. MS3 data were converted to “.ms3” format by pXtract within pFind Studio (version 2.8)26,27, and then analyzed by pFind

Comparative assessment of glycosylation of recombinant human ...(六)

(22) Wu, S. W.; Pu, T. H.; Viner, R.; Khoo, K. H. Novel LC-MS/MS product dependent parallel data acquisition function and data analysis workflow f

Chorioallantoic membrane grafting with chick embryo limb buds

ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chic

Denaturing Agarose Gel Electrophoresis of RNA

The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R

Dynabeads® Co-Immunoprecipitation Kit

实验概要The  Dynabeads® and buffers provided in this kit will enable you to a)  covalently immobilize antibodies of your choice onto the surface

Dynabeads® Co-Immunoprecipitation Kit

实验概要The  Dynabeads® and buffers provided in this kit will enable you to a)  covalently immobilize antibodies of your choice onto the surface

Construction and Manipulation of Large-Insert Bacterial Clone Libraries

Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In

Two-hybrid analysis of genetic regulatory networks2

2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey

Amino acid composition

There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi

Joint formation in chick limb bud CAM grafts

Abstract        Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive eve

第十七届北京分析测试学术报告会暨展览会(BCEIA2017)

  时间Dates:  学术报告会Conference 2017年10月09-11日 Oct.09-11, 2017  展 览 会 Exhibition 2017年10月09-11日 Oct.09-11, 2017  地点Location:北京•国家会议中心 China National Conve

peptide fingerprint mapping

Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension

In-gel digestion of proteins for peptide fingerprint mapping

Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension

Interleukin-6 Induced Acute Phenotypic Microenvironment Promote...(二)

As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of

Analysis of Proteins using Small Format 2D Gel Electrophoresis

Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei

Immunofluorescent Staining of Drosophila Larval Brain Tissue

INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblast

第五届金属组学国际研讨会会议日程及北京旅游攻略

  第五届金属组学国际研讨会会议日程 Scientific Programme   Wednesday, 9th Sept 2015   9:00  -21:00 Registration   Wednesday, 9th Sept 2015, Ginkgo Hall

AB SCIEX与GeneBio合作提高质谱库互动检索能力

SmileMS软件增强了 LC/MS/MS 系统对小分子的识别能力   生命科学分析技术的全球领先者——AB Sciex公司与Geneva Bioinformatics (GeneBio) SA 公司在今天宣布,他们将联手开发解决方案,以提高科学家快速筛选大量分子的能力。GeneBio公

Preparing Mitochondria from Rat Liver

Liver is a convenient source for functional intact mitochondria for a number of reasons. Animal tissue is more readily homogenized than plant tissue b

Construction of BAC Libraries:Megabase DNA Isolation

Megabase DNA IsolationMegabase-size DNA isolation from plantsTo construct large insert DNA libraries in BAC and YAC vectors, methods must be developed

FL3500双调制叶绿素荧光仪部分参考文献

FL3500双调制叶绿素荧光仪 (新升级型号为FL6000) FL3500双调制叶绿素荧光仪是专门用于对蓝绿藻或绿藻等微藻,叶绿体或类囊体悬浮物,乃至叶片进行光合作用研究的强大科研工具。仪器具备双通道测量控制,可控制测量样品的温度,并配备单翻转光(STF),内置多种可用户自行修改的测量程序

利用人工组合转录因子对人类基因组扫描-2

Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin

DNA抽提

DNA抽提(主要内容如下)·   Working with DNA·   DNA Extraction from Bacteria and Other Organisms·   DNA Extraction f

Centrifugation2

RELATIVE CENTRIFUGAL FORCE Figure F.2. Nomogram for R.C.F.Modern day ultracentrifuges can generate forces in excess of 300,000 times that of grav

Comparative assessment of glycosylation of recombinant human ...(一)

Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y

Waters在ASMS 2009上推出新型Synapt G2质谱系统

  Waters推出新型SYNAPT™ G2质谱仪—动力、功能以及速度要高于任何质谱系统         即时发布   2009年6月1日 费城   Waters公司(NYSE:WAT)今天宣布推出新一代功能强大的

蛋白质翻译后修饰的验证问题

Why are proteins, detected by mass spectrometry, not validated by site-specific antibodies?The modified motif could be detected by mass spectrometry (

胚胎干细胞和成体干细胞标志物

ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce

Comprehensive identification of novel proteins and N-glycosylation sites五

RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr

DNA的酶学操作

DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowl