eBioscienceELISA试剂盒使用手册

Q. 一步法Instant ELISA试剂盒，能做多少个检测？
How many tests can I perform with an Instant ELISA Kit?

Instant ELISA包含128 tests。 其中包含可以做8个点复孔标曲，共有32孔做标曲，96孔检测样本。
With an Instant ELISA, 128 tests (includes samples plus standards) can be performed. The number of tests results from the following composition:
One 96 well plate designed only for samples; 96 samples
Two 8-point standard curve in duplicates; 32 wells; thus the kit can be used at two different time points, each with their own standard curve.

Q. 我需要将标准品加到酶标孔中吗？
Do I have to add recombinant standard to the wells?

不需要。标准品已经冻干在酶标孔中，不需要再另外加入。
No, the recombinant standard is included as a component of the lyophilized reagents in the wells.

Q. 酶标孔中需要加二抗吗？
Do I have to add the secondary antibody to the wells?

不需要。二抗也已经被冻干在酶标孔中。
No, the secondary antibody is included as a component of the lyophilized reagents in the wells.

Q. 样本检测之前，需要稀释吗？
Do I have to dilute my samples before adding them to the wells?

A. 一般情况下，使用Instant ELISA检测，样本不需要稀释。但是具体请您参考随货说明书。In general, one can use undiluted samples with the Instant ELISA. Please refer to the product manual to determine if dilution of the sample is required for your kit of interest. The volume of sample required for the individual kits will also be listed in the product manual.

Q. 不同批次试剂盒，标准品的溶解体积一样吗？
Is the reconstitution volume of the standards the same for different lot numbers?

对大部分Instant ELISA 来说，标准品孔和空白孔的溶解体积是不一样的，因为它们批次特异性的。请您务必参考随货收到的产品说明书。
For some Instant ELISA kits, the reconstitution volume of the standard and blank wells may differ (these components are lot specific). Please use the manual provided in the Kit for specific volumes to use.

Q. 样本浓度如何计算呢？
How do I calculate my samples?

样本浓度稀释因子请参考具体说明书。
The calculation factor is always stated in the manual.