The available procedures for isolation and purification of photosystem I (PSI) from Chlamydomonas reinhardtii are time consuming and usually require several hours of sucrose gradient ultracentrifugation steps. This may lead to structural and functional impairment, including release of pigments and/or dissociation of protein subunits. Moreover, it is difficult to isolate intact complexes from thylakoids containing mutated PSI that accumulate to lower levels. Hence, isolation of intact PSI core complex depends on the speed of the procedure and the mildness of the extraction and purification. We have, therefore, modified the procedure for PSI isolation to both increase the yield of PSI and to reduce contamination by other pigment protein complexes. The modified procedure involves dodecyl maltoside solubilization of crude-thylakoid membranes followed by single-step column chromatography using a weak anion-exchanger. PSI eluted from the column between 13 mM and 15 mM Mg S04 . This new rapid purification procedure yielded pure PSI preparations with a Chl/P700 ratio of approx 90 and showing typical absorption difference spectra with a maximum bleaching occurring at 696 nm. Femtosecond transient absorption spectroscopy of purified PSI complex revealed a high degree of similarity in terms of excitation energy transfer within the PSI core to observations in cyanobacterial PSI.
从中国石化新闻办获悉,由中国石化总承包的马来西亚RAPID项目龙头装置1500万吨/年常压蒸馏装置(CDU)已于近日正式点火投产。据介绍,位于马来西亚国柔佛州边加兰地区的RAPID项目,是马来西亚国家......
分析测试百科网讯近日,岛津公司推出了NexeraPrep系列制备型纯化液相色谱仪(LC)。“制备工作”是从样品中分离和纯化特定物质的过程。该过程对于在制药工业中的药物发现期间提取目标化合物和杂质以及提......
【导语】计算机芯片中采用光刻技术创建图片来存储信息,光刻技术中紫外光和图片大小有成正比关系,紫外光波长越短,图片越小,短波紫外光条件非常苛刻而且昂贵。近期,JohnFourkas,来自美国马里兰大学化......
CobaltRapID空间位移拉曼光谱仪样本......