总结
活体多光谱荧光成像可以扣除组织自体荧光和进行多种荧光团成像。这可以增强信噪比并进行先进的多重荧光成像,实现更强大的研究设计。 参考文献 [1] Levenson RM, Lynch DT, Kobayashi H, Backer JM, Backer MV (2008). Multiplexing with multispectral imaging: from mice to microscopy. ILAR J 49-78. [2] Frangioni, JV (2003). In vivo near-infrared fluorescence imaging. Curr Opin Chem Biol.;7:626-34. [3] Weissleder R, Ntziachristos V (2003). Shedding light onto live molecular targets. Nat Med. 9( 1 ) 123-8. [4] Farkas DL, Du C, Fisher GW, Lau C, Niu W, Wachman ES, Levenson RM (1998). Non-invasive image acquisition and advanced processing in optical bioimaging. Comput Med Imaging Graph. 22(2):89-102. [5] Alexander L, Dhaliwal K, Simpson J, Bradley M. (2008) Dunking doughnuts into cells--selective cellular translocation and in vivo analysis of polymeric micro-doughnuts. Chem Commun (Camb). 14;( 30 ):3507-9 [6] Chou LY, Chan WC (2012). Fluorescence-tagged gold nanoparticles for rapidly characterizing the size-dependent biodistribution in tumor models. Unpublished.