WIKI资讯
WIKI资讯
PREPARE SOLUTIONS1. 0.25 M Sucrose Solution:Mix 40 g of sucrose (0.25M), 50 mL of 1M KH2PO4, pH 6.65 (0.1M), 2.5 mL of 1M MgCl2 (5 mM), and dH2O to 500 g. Filter through 0.45m. Add 1mL/mL of 1M DTT (added right before use). Store at 4oC2. 0.5 M Sucrose Solution:Mix 80 g of sucrose (0.25M), 50 mL of 1M KH2PO4, pH 6.65 (0.1M), 2.5 mL o......阅读全文
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA fro
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
蛋白质提取和纯化（主要内容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
· Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays in
Purification of plasmid DNA (miniprep) with high yields using diatomaceous earthKyung-Soo Kim and Charles K. PallaghySchool of Botany, La Trobe Univer
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants. &n
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia,
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove
实验概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are require
Top of pageProcedureOverviewSteps 1 - 8 Preparation of rat brain cytosolSteps 9 - 10 Culture of PC12 cellsSteps 11 - 30 Preparation of postnuclea
Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas
Organic substances.pKa and temperature dependence of pH for common buffers.ATP 0.1MBetaine 5MCresol red (Na) 50mMDTT 1M, 2.2M
实验概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (
CDNA文库（主要内容如下）· Construction of cDNA Library· &nbs