WIKI资讯
WIKI资讯
I. Purpose:A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up for short term culture of tumor cells. Care must be taken to avoid over-growth of normal fibroblast cells that are also present in the tumor specimen.II. Culture Procedure:A. Aseptic technique must be used when setting up the cultures, preferably under a laminar flow h......阅读全文
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome a
AbstractTumor genotyping can provide a useful guide to drive clinical trials, inform treatment options, and predict patient outcomes1. This is due, in
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
问：什么是肿瘤移植模型？ 答：人的肿瘤移植到小鼠体内构建的人源化肿瘤模型 问：这种模型意义在哪？ 答：筛药，为人民服务。（请为伟大的小鼠致敬！） 问：为啥要构建肿瘤模型，不能跳过直接上临床？不也可以筛药吗？ 答：嗯，好主意，要不你先来好不好？ .......
问：什么是肿瘤移植模型？答：人的肿瘤移植到小鼠体内构建的人源化肿瘤模型问：这种模型意义在哪？答：筛药，为人民服务。（请为伟大的小鼠致敬！）问：为啥要构建肿瘤模型，不能跳过直接上临床？不也可以筛药吗？答：嗯，好主意，要不你先来好不好？.......问：什么是CDX , PDX?答：急什么，认真听小编讲
I. Purpose:A: Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
实验概要 Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconc
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tub
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
I. Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. susp
肿瘤异质性是恶性肿瘤的特征之一，指的是肿瘤在生长过程中，经多次分裂增殖，子细胞呈现出分子生物学或基因方面的改变，从而使肿瘤的生长速度、侵袭能力、对药物的敏感性、预后等各方面产生差异。简言之同一肿瘤中可以存在很多不同的基因型或者亚型的细胞。因此同一种肿瘤在不同的个体身上可表现出不一样的治疗效果及预
肿瘤特异性染色体的错误分离通过保持肿瘤异质性来控制癌症的可塑性肿瘤异质性是恶性肿瘤的特征之一，指的是肿瘤在生长过程中，经多次分裂增殖，子细胞呈现出分子生物学或基因方面的改变，从而使肿瘤的生长速度、侵袭能力、对药物的敏感性、预后等各方面产生差异。简言之同一肿瘤中可以存在很多不同的基因型或者亚型的细胞。
癌细胞的基因组中到处都是突变（单一核苷酸的突变），有些突变或会通过激活癌基因的表达或关闭抑癌基因的表达而诱发癌症发生，然而可以说更重要的是在更大范围内肿瘤细胞中发生着基因组的异常，比如，诸如这样的细胞包含着异常数目的染色体（非整倍性），随着肿瘤不断进化，染色体的异常也会在毗邻的癌细胞之间发生变化
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid Culture of MSC from amniotic fluid1. Twenty amn
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
Primary referencestop American Journal of Clinical Pathology (AJCP), August 1975 to February 2006American Journal of Surgical Pathology
实验概要Rat neural stem cells (NSCs) serve as a well-established model for investigating human brain development, disease processes, and treat
实验概要Basic Methods of Culturing Drosophila实验步骤Stockkeeping1. Mechanics Most stocks can be successfully cultured by periodic
随着人类基因组计划（human genome project ）在2003年顺利完成，基因组测序技术取得了长足的进步，这直接导致了每兆基因组成本的大幅下降以及检测的基因组数量越来越多。人们对基因组的复杂性深感震惊，这也引导着测序技术的进一步发展。最近的一些突破性技术使得测序技术在更短的时间内可以
· Comparative Genomic Hybridization (CGH) CGH is a molecular Cytogenetic method of screening a tu
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
BCEIA2019 International Summit on Scientific Instruments Development--Registration in progressChina is one of the world’s fastest growing analytical a
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
流式细胞术 Flow cytometry CytoFLEX (Beckman Coulter Inc) was used to perform fluorescent expression analysis. Cells were harvested on the fol
IntroductionMulticolour 3D-FISH in combination with confocal microscopy, 3D image reconstruction and quantitative image analysis is an efficient tool