FlowCellAssayswithMicrotubules:Motility／DynamicsinFluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/depolymerizing factors on microtubules. Described here are some general procedures for flow cell assays.I. Solutions & SuppliesII. VE-DIC AssaysA. MotilityB. DynamicsIII. Fluorescence AssaysA. Oxygen ScavengingB. AssaysBack to protocolsI. Solutions & SuppliesBRB......阅读全文
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing
细胞组分和细胞器——细胞骨架
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin and Quantifying Labeling Stoi
细胞组分和细胞器——细胞器分离
Labeling Microtubules (Molecular Dynamics Inc. )Microtubules are involved in many aspects of cell motion including propulsion, mitosis, growth, and o
Vybrant® DyeCycle™ Ruby stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
Flow Cytometric Analysis of Cell Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
Yeast Cell Cycle by Flow Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
Dynamic Flow Assay in a Parallel Plate Flow Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit
实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is imp
LIVE/DEAD® Fixable Dead Cell Stain Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
Vybrant® DyeCycle™ Violet Stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy