实时定量PCR（RealtimequantitativePCR）
mRNA表达的研究 微小残留病变的检测 肿瘤耐药基因表达的研究 病毒感染的定量监测Vs普通PCR，RT-PCR的优势 采用封闭的检测模式，因此扩增产物导致污染的可能性比普通PCR要小得多 。 扩增产物的检测在PCR扩增过程中同时进行，并且数据的采集、分析全部由 仪器 自动完成，因此整个检测所 需的时间比普通PCR要节省许多。 检测模式功能强大，具备定性、定量、突变、多项目等检测功能。而普通PCR要完成上述项目需采用不同的技术平台。 进行定量检测时，其定量线性范围（5-6 logs）比普通PCR（2-3 logs）要宽得多。 Real-time定量PCR仪是在普通PCR 仪的基础上再配备一个激发光源和荧光信号检测系统的装置。通过PCR反应的数学函数关系，加入标准品，可以对待测样品中的目标基因进行准确定量。Real-Time PCR 热循环仪（PCR仪）荧光检测系统计算机及软件系统......阅读全文
Quantitative－PCR
实验概要Quantitative PCR involves co-amplification of two templates: a constant amount of a preparation containing the desired target sequence and var
SemiQuantitative－RTPCR
The RT-PCR method can be used not only to detect specific mRNAs but also to semi-quantitate their levels. Thus, one can compare levels of transcripts
SYBR－Green－Quantitative－PCR－Protocol
SummaryQuantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the
A－rapid,－quantitative－and－inexpensive－method－for－detecting－apoptosis
ASTRACTWe describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell
Quantitative－Determination－of－Peptides－by－Sulfhydryl－(SH)－Groups
Quantitative Determination of Peptides by Sulfhydryl (-SH) GroupsAuthor: David Van Horn, Greg BulajSource: Contributed by David Van Horn, Dept. of Che
SemiQuantitative－Measurement－of－Proteins－by－Dot－Blotting
Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if y
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－4
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－5
3. Characterization of the method precision and repeatability.a. Ensemble PCRs in the same conditions established before using quantities of target in
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－3
Competitive RT-PCR in Different Tilapia TissuesAbundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantita
A－rapid,－quantitative－and－inexpensive－method－for－detecting－apoptosis2
Figure 1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (% apop
实时定量PCR（Realtime－quantitative－PCR）
mRNA表达的研究 微小残留病变的检测 肿瘤耐药基因表达的研究 病毒感染的定量监测Vs普通PCR，RT-PCR的优势 采用封闭的检测模式，因此扩增产物导致污染的可能性比普通PCR要小得多 。 扩增产物的检测在PCR扩增过程中同时进行，并且数据的采集、分析全部由 仪器 自动完成，因此整个检测所 需的时
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－1
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－2
Determination of Accuracy of the Competitive PCRTo test the precision of the results obtained with this competitive PCR, five different amounts of T (
定量RTPCR－（Quantitative－RTPCR）
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of mRNA expres
半定量RTPCR－（SemiQuantitative－RTPCR－）
RT-PCR AnalysisSolutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1% Triton X-10025 mM MgCl2use at a concentration of 1.5 mMLysis Solutio
实时荧光定量PCR（Quantitative－Realtime－PCR－，qPCR）技术介绍
荧光定量PCR也叫Real-Time PCR，是美国PE（Perkin Elmer）公司1995年研制出来的一种新的核酸定量技术。荧光定量PCR的发展史是一部ABI、罗氏和伯乐等巨头的荡气回肠的斗争史，感兴趣的可以去查查。该技术是目前最成熟，使用最广泛的半定量PCR技术。 荧光染料法（SYBR
RealTime－or－Kinetic－PCR
The DNA Facility houses the “real-time” or kinetic PCR instrument, the Applied Biosystems Model 7700 sequence detection system (the TaqMan instrument)
Multiple－studies－with－a－single－experiment:－The－Power－of－...（六）
•SCX and high pH reversed phase fractionation are both orthogonal to low pH C18 LC separation•Strong cation exchange (SCX) requires sample desalting
Multiple－studies－with－a－single－experiment:－The－Power－of－－...（一）
Multiple studies with a single experiment: The Power of Quantitative Multiplexing Multiple studies with a single experiment: The Power of Quantitati
Waters推出Xevo－G2－TOF台式飞行时间质谱
Waters Corporation. (1/10/11). "Press Release: Waters Combines Best-in-Class Performance, Versability and Usability Technologies in New Xevo G2 Tof Be
qpcr和ddpcr基因检测的区别
QPCR的英文全名是Real-time Quantitative PCR Detecting System。即实时荧光定量核酸扩增检测系统，也叫实时定量基因扩增荧光检测系统，简称QPCR。基因扩增热循环仪+荧光仪+终点定量试剂，构成PCR-DNA终点法定量检测（End-point quantitat
德国IBL－ASP－ELISA试剂盒使用说明（一）
ASP (Domoic acid)direct ELISA Enzyme immunoassay for the quantitative determination of amnesicshellfish poison (Domoic acid) in algal, shellfish or se
AB－SCIEX欢迎蛋白质组学者们参加第10届HUPO会议的技术交流会
第10届HUPO会议将在2011年9月4-7日于瑞士日内瓦举行，届时，AB SCIEX公司将举办技术讲座交流会。欢迎来自中国和全球各地的科学工作者们注册：Register for our workshopsSeptember 4, 2011 -
Real－Time－PCR－Primer－Sets
Real Time PCR Primer SetsNOW OVER 300 PRIMER SETS!!!UPDATED: NOVEMBER 10th, 2003Quantitative RT-PCR is an important step for the validation of express
HbsAg和HBVe抗原定量检测的临床意义
贾继东 李海 作者单位100050首都医科大学附属北京友谊医院肝病中心【摘要】HbsAg和HBVe抗原（HBeAg）定量检测的发展有助于检测检测患者血中HBV抗原的动态变化，在干扰素治疗过程中优于单纯检测HBV DNA。长效干扰素治疗12和24周，HbsAg水平及其下降幅度与患者治疗结束
RTPCR、QPCR、Realtime－PCR、realtime－RTPCR你真的区分的开吗？
多少同学能将 RT-PCR、Realtime-PCR、QPCR区分开？有多少同学还在犯晕？今儿这贴，师兄就带你区分区分，拨开那扇 PCR 迷雾。什么是 RT-PCR？RT-PCR就是逆转录 PCR（reverse transcription PCR）或者称反转录PCR(reverse transcr
实时荧光定量PCR检测系统的介绍
实时荧光定量PCR检测系统也叫实时荧光定量核酸扩增检测系统（英文全名是Real-time Quantitative PCR Detection System）,简称实时荧光qPCR,qPCR的核心是实时荧光定量PCR仪及与其配套的检测分析软件系统。聚合酶链式反应（Polymerase Chain
分子生态学词汇数量性状
中文名称：数量性状外文名称：quantitative characters释 义：一个群体内的表现连续变异的性状
液相色谱仪色谱曲线相关术语定量分析
定量分析(quantitative analysis)为测定试样中各种成分(如元素、根或官能团等)的合量而进行的分析。
三种国产乙型肝炎病毒基因荧光定量试剂检测结果分析
［摘 要］ 目的：探讨上海复星、深圳匹基、上海科华三种国产乙型肝炎病毒基因(HBVDNA)荧光定量试剂的临床检测结果相互之间是否存在差异。方法：将相同的标本分别用三种国产HBVDNA荧光定量试剂进行HBVDNA荧光定量测定。然后再对检测结果进行对比，分析相互之间是否存在差异。结果：23例已知的慢性