WIKI资讯
WIKI资讯
The DNA Facility houses the “real-time” or kinetic PCR instrument, the Applied Biosystems Model 7700 sequence detection system (the TaqMan instrument). The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. As little as a single copy of a particular sequence can be specifically amplified and detected. Theoretically, there is a quantitative relationship between amount of starting target s......阅读全文
Lot-No.Ref. FR342 Expiry time: 1 year100 Tests (Ready to use PCR kit) -Only for in vitro use--Only for research use – To be used by a
Lot-No.Ref. FR340 Expiry time: 1 year100 Tests (Ready to use PCR kit) Zika Virus (Real time) DOUBLE CHECK &n
2013年5月16日-19日，由中国化学会主办、厦门大学承办、复旦大学、浙江大学协办的为期四天的第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议在美丽的海滨城市厦门隆重召开。以下是微全分析专场报告。上海交通大学
MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2aMicroRNAs are positive and negative r
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
聚合酶链式反应(Polymerase Chain Reaction，PCR)可对特定核苷酸片断进行指数级的扩增。在扩增反应结束之后，我们可以通过凝胶电泳的方法对扩增产物进行定性的分析，也可以通过放射性核素掺入标记后的光密度扫描来进行定量的分析。无论定性还是定量分析，分析的都是PCR终产物。但是在许多
慢性肾病病人一般会出现很多的并发症，其中大多数肾损伤晚期病人都患有严重的高血压。盐是慢性肾病和高血压发病的主要致病因素之一，但其中的病理机制仍然不清楚。本文利用蛋白质组和磷酸化蛋白质组联合分析的技术，对慢性肾病并发高血压的病理机制进行了全面系统性的分析。 Proteomic and p
慢性肾病病人一般会出现很多的并发症，其中大多数肾损伤晚期病人都患有严重的高血压。盐是慢性肾病和高血压发病的主要致病因素之一，但其中的病理机制仍然不清楚。本文利用蛋白质组和磷酸化蛋白质组联合分析的技术，对慢性肾病并发高血压的病理机制进行了全面系统性的分析。 Proteomic and p
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is p
一滴残留在裙子上的精液使得美国总统Bill Clinton不得不坦承他与白宫实习生有不正当的关系。因为他知道现在的生物科技就连一个精子也能被用来做为证据。这种将极微量的生物标本化为可供鉴定的现代技术正是PCR(Polymerase chain reaction)--聚合
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle Numbe
PCRPolymerase Chain Reaction1) Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul templ
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
For ionisation to take place at all, chemical reaction between the sample and the&nbs
What is degenerate PCR? Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polym
Non-denaturing PAA gelsTo separate PCR products differing in only a few bp in length (for example, microsatellite markers), 6-10% PAA gels need to be
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
3. Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA p
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
3’ Adaptor Ligation and PurificationHeat shock the RNA by putting at 90°C for 30 seconds. Snap cool on ice.Set up the 3’Adaptor ligation reaction in a
实验概要 We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter se
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
实验概要 Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and g
Influence of annealing temperature and number of loci amplifiedLike any other PCR, multiplex reactions should be done at a stringent enough temperatur
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles. COMPONENTVOLUMEFINA
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec