WIKI资讯
WIKI资讯
We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneous precipitation and denaturation with TCA and 2ME in cold acetone. We also describe protein solubilization prior to IEF, either in classical rod gels or in IPGs, using two different solutions. The procedure is easy to carry out. The major caveats are (1) keep sam......阅读全文
实验概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
A Method for Micro-Scale Isolation and Purification of GangliosidesStephan Ladisch~Director, Center for Cancer and Transplant Biology, Children's
实验概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have
实验概要The Dynabeads® and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de
Overview1) Purpose: Data Independent Acquisition (DIA) has been applied for large-scale protein and PTM quantification on the Thermo Scientific ™
Digestion of Proteins and Extraction of Peptides from an Acrylamide GelSherry Niessen, Ian McLeod and John R. Yates IIIDepartment of Cell Biology, The
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
FL3500双调制叶绿素荧光仪 （新升级型号为FL6000） FL3500双调制叶绿素荧光仪是专门用于对蓝绿藻或绿藻等微藻，叶绿体或类囊体悬浮物，乃至叶片进行光合作用研究的强大科研工具。仪器具备双通道测量控制，可控制测量样品的温度，并配备单翻转光（STF），内置多种可用户自行修改的测量程序
INTRODUCTIONIn many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix.
第一天(2013年6月6日)13:45-14:30 展商技术论坛 1： 会场：宴宾厅 II，2楼 议题：Cell-based assays and 3D cell culture：Your body is in 3D – Why is your cell cult
IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into
1. Excision of protein bands (spots) from polyacrylamide gelsRinse the gloves you use with water to avoid traces of dust in your sample.Rinse the gel
RNA extraction with TRIzol (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total RNA extractio
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well). For growth in minimal media, 1 ml of a saturated o
Introduction The most important aspect of our cloning vectors is that t
Large Scale Preps: (See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
Overview TROPONINS The calcium-dependent regulatory protein complex located on the thin actin filaments of muscle comprises of TnC (17
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke and Michael B. YaffeCenter for Cancer Research, Massachusetts Institute
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom