RealspaceandrealtimedynamicsofCRISPRCas9visualizedby...（一）
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopyAbstractThe CRISPR-associated endonuclease Cas9 binds to a guide RNA and cleaves double-stranded DNA with a sequence complementary to the RNA guide. The Cas9–RNA system has been harnessed for numerous applications, such as genome editing. Here we use high-speed atomic force microscopy (HS-AFM) &nbs......阅读全文
Real-space and real-time dynamics of CRISPR-Cas9 visualized by ...（一）
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopyAbstractThe CRISPR-associated endonuclease Cas9 bind
Real-space and real-time dynamics of CRISPR-Cas9 visualized by ...（三）
Fig. 4Structural rearrangement of the HNH domain. a, b HS-AFM images of the HNH domain in the high-height (a) and low-height (b) states. The mean ce
Real-space and real-time dynamics of CRISPR-Cas9 visualized by ...（四）
Fig. 6HS-AFM observations of the non-specific transient binding of Cas9–RNA. a Sequential HS-AFM images of Cas9–RNA molecules transiently bound to
Real-space and real-time dynamics of CRISPR-Cas9 visualized by ...（二）
ResultsRNA-induced structural stabilization in Cas9We first observed apo-Cas9 and pre-assembled Cas9–RNA on a mica surface treated with 3-aminopro
Real-space and real-time dynamics of CRISPR-Cas9 visualized by ...（五）
Correlation analysis of HS-AFM images2D correlation coefficients were calculated between the HS-AFM images of the first frame and each of the fram
Exercise 12.9 - Eukaryote Growth Dynamics
Exercise 12.9 - Eukaryote Growth Dynamics LEVEL II Materials Suspension cultures set up from Exercise 12.8 Sterile transfer pipettes
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing
AKAP95 role in mitosis and chromosome dynamics
The chromatin packaging of the genome is dynamic, changing with the cell cycle and with transcriptional regulation. During mitosis, chromatin is conde
Real-time PCR 数据导出 —— ABI仪器篇（一）
1. Real-time PCR数据：Real-time PCR完成后，所有数据并非可以直接导出，由于每一次实验的情况都不相同，仪器的很多默认设定都会对最终数据结果造成重大偏差，所以在完成PCR过程后，需要人为对实验结果进行条件设置，才能提取到科学有效的实验结果，进而完成对实验结果的分析和判断。
Real-time PCR
实验概要The exponential amplification via reverse transcription polymerase chain reaction provides for a highly sensitive technique in which a very low