WIKI资讯
WIKI资讯
triple quadrupole liquid chromatography tandem mass spectrometry method is presented for the quantitative determination and confirmation of melamine residues in catfish. Catfish tissue was extracted with 50:50 acetonitrile:water and 1 N hydrochloric acid and cleaned-up using Oasis® MCX solid phase extraction cartridges. Extracts were analyzed by Thermo TSQ LC-MS-MS with HILIC chromatography and electrospray ion......阅读全文
近期关于亚洲国家发生的三聚氰胺污染婴幼儿配方奶粉的新闻报道，又一次引起了全球对食品供应链安全的关注。其实，2007年3月相类似的事件在美国宠物食品中也曾发生过，世界各国政府管理机构都在研究科学的质控方法检测三聚氰胺和其代谢物，以其保证人类食品和动物饲料的安全性。 有关婴幼儿配方奶粉、饼干和糖果中检
一、液相色谱——串联质谱法测定三聚氰胺 美国国家食品安全与技术中心建立了测定食品中的三聚氰胺的新方法 TSQ Quantum系统在三聚氰胺的分析上得到了应得的认可。我们可以很容易的在含有10ppb三聚氰胺的鲶鱼的分析中，获得超过3800的信噪比。 >>下载全文 二、ELISA方法检
实验概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
生物分子发表的代表性文献 QTRAP：同时具有三重四极杆和线性离子阱性能的独一无二的LC/MS/MS系统 QTRAP系统最早在ASMS 2002上，作为第一台商用的线性离子阱发布，是世界上唯一的线性离子阱和三重四极杆的复合
据悉：根据国际食品科学院(International Academy of Food Science and Technology，IAFoST)近日公布的消息，我国国家食品安全风险评估中心技术总师，重点实验室主任，WHO食品污染监测合作中心(中国)主任吴永宁研究员当选该院院士(Fellow)。
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
E.M. PROCESSING SCHEDULE - EPOXY RESINFix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue shou
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
To determine the minimum dose of Katushka plasmid needed to give detectable fluorescent intensity, we decreased the amount of pTurboFP635 to 0.5 and 1
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
Quantitative Determination of Peptides by Sulfhydryl (-SH) GroupsAuthor: David Van Horn, Greg BulajSource: Contributed by David Van Horn, De
CD SpectroscopyCD spectra for peptides and reference solutions were recorded at 298 K on a Jasco J-920 CD spectrometer with a 1-mm quartz cuvette
Megabase DNA IsolationMegabase-size DNA isolation from plantsTo construct large insert DNA libraries in BAC and YAC vectors, methods must be developed
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
小鼠白色脂肪、棕色脂肪及脂肪肝含量的CT测量研究选自PLoS ONE, May 2012 研究背景：现代的生活方式导致了肥胖的高发。由能量摄取和消耗不平衡所导致的肥胖通常会伴随高血压，血脂异常，冠心病等症状，最终导致新陈代谢异常。脂肪的分布而不是总脂肪量决定了新陈代谢的状况。皮下脂肪对人体
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Exercise 12.10 - Establishment of a Primary CultureLEVEL IIIMaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scis
Determination of Accuracy of the Competitive PCRTo test the precision of the results obtained with this competitive PCR, five different amounts of T (
ConclusionsQuorum-sensing signaling systems involving the interaction between a signaling peptide and its cognate histidine kinase receptor are widely
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
实验概要Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, m
（选自PLoS ONE, May 2012）LCT200具备内置的棕色脂肪测量的标准操作方法（protocol），检测体内棕色脂肪的分布和定量体积，该技术已获得欧洲及美国的专利，比使用同位素的方法安全。研究背景：现代的生活方式导致了肥胖的高发。由能量摄取和消耗不平衡所导致的肥胖通常会伴随高血压，血脂
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
Bradford – Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 µl p
2009年4月27日，布鲁克道尔顿公司推出新一代ultrafleXtreme MALDI-TOF/TOF质谱仪，这是市场上唯一的，在TOF和TOF/TOF模式下都能获得真正的1 kHz采样速度的仪器，可用于广泛的研究、临床、和应用蛋白质组学。 布鲁克的新型ultrafleXtreme质
LC-MS data processingN-glycan data were processed using UNIFI 1.7 with Glycobase 3+ (Waters Corporation, Milford, MA) for N-glycan structure. The pe
Cuvette holders in the sample chamber of the spectrophotometer were thermo-controlled at 25°C. For the blank test, 100 ml of 50 mM potassium phosphate