WIKI资讯
WIKI资讯
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type 5 DNA and Epstein-Barr virus nuclear antigen 1, allowing episomal amplification of plasmids containing the viral EBV origin of replication. This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line us......阅读全文
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
实验概要293fectin™ is a proprietary, cationic lipid-based formulation for transfection of DNA into eukaryotic cells. 293fectin™ is optimized f
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from confluent T-flasks or from suspension cultures at h
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
PurposeTransient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) pro
Author: Nanci DonackiSource: Contributed by Nanci DonackiAbstract: Procedure for establishing hybridoma in one stepReagents(StemCell Te
ReagentsMedium A - Pre-fusion Medium and Hybridoma Expansion MediumMedium B - Fusion Medium Medium C - Hybridoma Recovery MediumMedium D - Hybridoma S
实验概要将转移基因整合到细胞染色体DNA上,形成稳定表达转移基因的细胞系。 实验原理 细胞转染技术是目前广泛应用于病毒基因结构与功能以及基因调控等的研究。细胞转染可分为短暂转染和稳定(或永久) 转染两种。在短暂转染中,被转染基因并不整合至细胞染色体中,因而不能随细
常规操作(主要内容如下)· Aseptic Technique· Culture Ves
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
Seeding After cells are thawed:NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being car
实验概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
3. If your result falls into any quadrant other than the "High Yield-High Viability" quadrant, refer to Appendix D, Improving Cell Yield and
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI™, USA) were initially thawed and pre-cultivat
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach s
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and François-Loïc CossetAdapted from Gene Tra
选择何种稀释液稀释DNA及转染试剂，对于制备有效的转染复合物至关重要。除了温度，孵育时间外，稀释液的性质对于制备高效的转染复合物亦非常重要，同样影响DNA转染效率。根据我们的实验数据，使用合适的稀释液得到的转染效率是使用错误稀释液转染效率的至少50倍。更为重要的是，实验者总是忽视稀释液的重要性，甚至
Tissue collection1. Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2. All wome
Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14 2002Date Modified: Tue
Growth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampic
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
Electroporation ProtocolPreparation of Electro-competent Cells:1. Grow XL1-Blue cells on a tetracycline plate (20 ug tet/ml of LB agar)2. Inoculate 3
DNA转染· Transfection of Mammalian Cells Using Lipofectamine (LTI)· &n
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med