WIKI资讯
WIKI资讯
Asymmetric sequences are indicated by *.Single letter code:R = G or A; Y = C or T; W = A or T; M = A or C; K = G or T; S = C or G;H = A, C or T;V = A, C or G;B = C, G or T;D = A, G or T;N = G, A, T or C. RecognitionsequenceEnzymeAAT^ATTSspIAGC^GCTEco47IIIAG^CTAluIAGG^CCTEco147IAGT^ACTScaIATTT^AAAT SmiICACGTC(-3/-3)^*BtrICAC^GTGEco72ICACNN^NNGTGOliICAG^CTGPvuIICAYNN^NNRTGMslICCC^GGGS......阅读全文
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowl
PCRPolymerase Chain Reaction1) Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul templ
Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D. *Methods and reagents is a unique monthly column that highlights current discussion