WIKI资讯
WIKI资讯
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition of a motile phenotype is one requirement for a cell to become metastatically competent. Procedure1.Place the chamber in 6 well tissue culture plate. Plate 1 million cells in the top chamber of noncoated polyethylene teraphthalate membranes (6-well insert, pore size ......阅读全文
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays.Solutions and Reagents Freshly removed or
3.2.1 LabelingThis method requires the starting material 3′-amino-3′-deoxyATP (15).1. Activate 7-diethylaminocoumarin-3-carboxylic
Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin