Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistration of the drug and monitoring the depth of anesthesia. Supportive care may be needed. Maintenance of injectable anesthesia can be through repeated bolus doses of the drug or through a constant infusion. Infusion rates are calculated based on the clearance time of the ......阅读全文
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pa
BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized
Finite element computational modeling of the resistive heating during the maximum exposure (150 V, 100 μs/phase, 10 Hz, 30 s) de
Intraperitoneal:Equipment: Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume: The volume injected IP into a
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
中国P4实验室项目以现有中科院武汉病毒所为依托建设,一期投资近1亿元人民币。2018年1月5日,武汉P4实验室正式运行。新落成的P4实验室位于武汉市江夏区的中科院武汉病毒所郑店园区内。P4实验室是目前人类所拥有生物安全等级最高的实验室,也是专用于烈性传染病研究的大型装置,像埃博拉病毒这样的对人体具有
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
实验概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal® CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
实验概要Enrich untouched Dendritic Cells (DCs) by depleting T cells, B cells, monocytes/ macrophages, NK cells, erythrocytes and most granuloc
表3:大鼠和小鼠使用不同的镇静剂药物(IP=intraperitoneal; SC=subcutaneous; PO=per os(by mouth); IV=intravenous)药物大鼠剂量(mg/kg)小鼠剂量(mg/kg)注解Tranquilizers/sedativesAcepromaz
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
2. 参考文献/ 出处1. Rogers, J. V., C. L. Sabourin, Y. W. Choi, W. R. Richter, D. C. Rudnicki, K. B. Riggs, M. L. Taylor, and J. Ch a ng.2005. De
实验概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for t
实验概要 The ChargeSwitch® gDNA Mini and Micro Tissue Kits allow rapid and efficient purification of genomic DNA from mini (10-25 mg) or
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and
众所周知,2017 诺贝尔生理或医学奖颁发给了三位美国遗传学家杰弗里·霍尔(Jeffrey C. Hall)、迈克尔·罗斯巴什(Michael Rosbash),以及迈克尔·杨(Michael W. Young),以表彰他们在发现果蝇生物节律分子机制方面的贡献。而在此前,医学界真正将生物节律——
实验概要The ChargeSwitch® PCR Clean-Up Kit allows rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-n
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first