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GuidelinesfortheUseofAnalgesicsandTranquilizersinLaboratoryAnimal5

Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistration of the drug and monitoring the depth of anesthesia. Supportive care may be needed. Maintenance of injectable anesthesia can be through repeated bolus doses of the drug or through a constant infusion. Infusion rates are calculated based on the clearance time of the ......阅读全文

Yale University Policy for Aseptic Amphibian Survival Surgery

Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the

Guidelines for the Use of Analgesics and Tranquilizers in Laboratory Animal

What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pa

Guidelines for theUse of Analgesics and Tranquilizers in Laboratory Animal3

BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a

Guidelines for Aseptic Rodent Survival Surgery

Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e

Experimental Surgery-2

Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized

医学研究:电刺激可帮助控制大鼠出血(三)

Finite element computational modeling of the resistive heating during the maximum exposure (150 V, 100 μs/phase, 10 Hz, 30 s) de

Blood collection and administration of fluids and drugs (mouse)-2

Intraperitoneal:Equipment: Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume: The volume injected IP into a

Guidelines for theUse of Analgesics and Tranquilizers in Laboratory Animal2

GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o

通过细胞受体代谢生物素化进行图像分析

Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar

全球高危P4实验室简介以及当地的人口数字

中国P4实验室项目以现有中科院武汉病毒所为依托建设,一期投资近1亿元人民币。2018年1月5日,武汉P4实验室正式运行。新落成的P4实验室位于武汉市江夏区的中科院武汉病毒所郑店园区内。P4实验室是目前人类所拥有生物安全等级最高的实验室,也是专用于烈性传染病研究的大型装置,像埃博拉病毒这样的对人体具有

Light Microscopy

The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool

Activation and Expansion of Human Treg Cells

实验概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal® CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp

FDA刚重磅发布《Bioanalytical Method Validation Guidance for Industry》

   I. INTRODUCTION   引言   This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),

Human DC Enrichment Kit

实验概要Enrich  untouched Dendritic Cells (DCs) by depleting T cells, B cells,  monocytes/ macrophages, NK cells, erythrocytes and most granuloc

D-荧光素钾盐/钠盐体内使用手册(四)

表3:大鼠和小鼠使用不同的镇静剂药物(IP=intraperitoneal; SC=subcutaneous; PO=per os(by mouth); IV=intravenous)药物大鼠剂量(mg/kg)小鼠剂量(mg/kg)注解Tranquilizers/sedativesAcepromaz

Complete Mouse Necropsy

EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th

过氧化氢蒸汽 ( H P V ) 生物有效性(二)

2.  参考文献/  出处1. Rogers, J. V., C. L. Sabourin, Y. W. Choi, W. R. Richter, D. C. Rudnicki, K. B. Riggs, M. L. Taylor, and J. Ch a ng.2005. De

Magnetic Depletion of SSEA-4+ Undifferentiated Embryonic Stem Cells

实验概要Human  embryonic stem cells can be differentiated into neural-, mesenchymal  and hematopoetic stem cells. This product is intended for t

Isolation of Genomic DNA from Tissue Using ChargeSwitch® Technology

实验概要 The ChargeSwitch®  gDNA Mini and Micro Tissue Kits allow rapid and efficient purification  of genomic DNA from mini (10-25 mg) or

The Impact of Harmo... (一)

实验概要During more than  25 years of application in immunological sciences, ELISPOT has been  established as a routine, robust, versatile, and

Cell Metabolism | 医学的第四维——生物节律

  众所周知,2017 诺贝尔生理或医学奖颁发给了三位美国遗传学家杰弗里·霍尔(Jeffrey C. Hall)、迈克尔·罗斯巴什(Michael Rosbash),以及迈克尔·杨(Michael W. Young),以表彰他们在发现果蝇生物节律分子机制方面的贡献。而在此前,医学界真正将生物节律——

High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up

实验概要The ChargeSwitch®  PCR Clean-Up Kit allows rapid and efficient purification of PCR  products from salts, primers, dNTPs, and other non-n

Preparation of Mitochondria from Rat Liver

Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl

Guidelines for theUse of Analgesics and Tranquilizers in Laboratory Animal6

 Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas

Genomic DNA Extraction - PureLink™

实验概要The  PureLink™ Genomic DNA Purification Kit allows rapid and efficient  purification of genomic DNA. The kit is designed to efficiently

Taq酶PCR实验方法介绍

General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec

PfuDNA聚合酶PCR实验方法介绍

General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec

Principles of Aseptic Technique

INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce

基本无菌化技术3

PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first

Principles of Aseptic Technique——3

PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first