WIKI资讯
WIKI资讯
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soften the fruit tissues as the fruit ripens, making it even more attractive to animals that might disperse the seeds. So perhaps the activities of these proteases enzymes will increase during the ripening process.Applications of plant proteases, such as ‘bromelain’ from the......阅读全文
Chloroplasts are metabolically important organelles that perform many essential functions within plant cells. The chloroplasts can be subdivided i
实验概要We provide a general IP procedure including a list of reagents and a table to help you choose the correct protein beads.Immunoprecipit
Table 1: Hydrolysis of 125I-labeled Ub-PESTc by the purified YUH1.Specific activity againstDUBsCbz-LRGG-AMC125I-labeled Ub-PESTcYUH13.2 x 10-105.
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of se
借助Chromotek公司GFP-Trap如何设计成功的IP实验？How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap®PreambleThis document
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP)
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions
MTT Assay on Reattached CellsAs seen in Fig. 2 , the proportion of viable MSC that re-attached was significantly higher (p
Bovine Ig(Total Immunoglobulin) ELISA KitCatalogue No: EB0001 Size: 48T/96T Reactivity: BovineDetection Range: 1.563-100ug/mlSensitivity: <0.938ug/
为了进一步提高检测基因的效率，我们对萤光素酶基因序列的密码子进行了优化，使得它在多种哺乳细胞中的表达水平提高了5～10倍；同时，为了减少对基因的非特异性调控，我们也对萤光素酶的载体进行了优化，去除了载体上哺乳动物转录因子结合序列的保守序列，从而大大降低了实验的本底，显着提高了实验的相对信号强度。优化
随着发光(luminescence)技术在多种生物实验中的广泛应用，生物发光(bioluminescence)技术越来越成为首选的生物检测手段。在这篇文章中，我们将详细讨论生物发光技术在生物检测中的应用，以及它与其它发光检测手段相比所显示出的优点。 1 生物发光的特点 根据产生光子的
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
实验概要The procedure presented below describes a method for preparing rat liver.主要试剂1. Aluminum Foil2. &nb
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g for 5 min
SDS-PAGE胶样品排列： MarkerUII 37CUI’I’ Marker：低分子量蛋白marker，上样10ul
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from confluent T-flasks or from suspension cultures at h
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia,
实验概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
1) After ECL development, wash membrane once for 10min with PBST.2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
NUCLEAR EXTRACTION OF TISSUE CELLSThis procedure was developed for HNEK cells grown in KGM, from Clonetics. All steps are performed on ice, with
Preparation of cell lysate:1. For cells in suspension, grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 µl p
实验概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have
实验概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要试剂Hypotonic Buffer Solution20 mM