DegeneratePCR
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequence, you use mixed PCR primers. That is, if you do not know exactly the sequence of the gene you are going to amplify, you insert "wobbles" in the PCR primers where there is more than one possibility. For instance, if you just have a protein motif, you can back......阅读全文
Degenerate PCR
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
Degenerate PCR, a short guide.
What is degenerate PCR? Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using specific PCR pr
其它PCR方法
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in detailRecommended Reagent ConcentrationsRecommend
TAIL PCR Protocol
TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 react
Competitive RT-PCR Strategy for Quantitative Evaluation -1
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I Quantiza
递减聚合酶链式反应的简介
递减PCR，亦称降落PCR(touchdown PCR)是一种PCR（聚合酶链式反应）方法，用来避免非特异性序列的扩增。PCR中引物的黏合温度(annealing temperature)决定了黏合的特异性，温度越高特异性越强，但过高则不能实现引物和模板的结合，而过低会产生大量非特异性产物。因此
Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR
1) Immunocapture stage Coating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6). Extraction buffer: (20 mM Tris-HCL (pH 8
Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR
1) Immunocapture stage Coating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6). Extraction buffer: (20 mM Tris-HCL (pH 8
CORE SAMPLE PCR
A method to re-PCR unique bands from products of mixed sizeContentsINTRODUCTIONPROTOCOLCOMMENTSINTRODUCTIONThe products of a PCR reaction - especially
"CORE SAMPLE" PCR: A method to re-PCR unique bands from products of mixed s
INTRODUCTION The products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - often cont