WIKI资讯
WIKI资讯
Glucosamine Rapid AssayMRTHOD:Place sample (containing 0.5 - 10 µg GlcN) in a Pyrex screw capped tube.Add HCl to a final concentration of 2N and a final volume of 0.6 ml. Flush with nitrogen.Stopper tightly and hydrolyze 16h at 100oC.Prepare standards containing 0 - 20 µg GlcN from a stock solution of 1.5 mg/ml. (Use 0, 3, 5, 8, 10, 12 ml of stock solution and add water to make up to 300 µl. Then add 300 µl 4N HCl to......阅读全文
跳值历史背景自1999年，Abbott AxSYMTM Troponin I项目中首次报道False Increases of Troponin I attribute to incomplete separation of serum，Clinical Chemistry 45, No. 5, 1
2 目前国内外应用于检测耐药结核杆菌的分子生物学方法随着分子生物学的发展，尤其是PCR技术的问世，检测耐药可以直接从基因入手，这样与传统的结核杆菌药敏试验相比，不仅大大缩短了检测周期，实现了自动化，而且也降低了生物实验室的危险性。耐药检测包括用PCR扩增基因组内携带的耐药性区域，和进行扩增产物的突变
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
三、线粒体膜势能的检测 线粒体在细胞凋亡的过程中起着枢纽作用，多种细胞凋亡刺激因子均可诱导不同的细胞发生凋亡，而线粒体跨膜电位DYmt的下降，被认为是细胞凋亡级联反应过程中最早发生的事件，它发生在细胞核凋亡特征（染色质浓缩、DNA断裂）出现之前，一旦线粒体DYmt崩溃，则细胞凋亡不可逆
Bradford – Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster
郝繁运 综述 中国误诊学杂志 2007年 第一期 第五次全国中青年检验医学学术会议论文汇编 摘要：本文主要介绍了酶免疫分析的发展现状，从酶免疫分析标记技术若干环节的技术进步、在方法学上与现代化技术的融合与发展、酶免疫分析技
Figure 1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (%
Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observ
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
实验概要The ChargeSwitch® gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. Afte
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In&n
实验概要Alanine Transaminase (ALT) is a transaminase (EC 2.6.1.2) also called serum glutamic pyruvic transaminase (SGPT). Alanine Transaminase
Or gel mobility shift assay, gel shift assay, gel retardation, electrophoretic mobility shift assay (EMSA) EMSA Using Oligos (Mike A. Dyer)A
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a po
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
基于epMotion 5075t系统与KPPA HyperPlus试剂盒的全自动测序前文库制备方案Automated KAPA HyperPlus DNA Library Preparation for Illumina® Sequencing on the Eppendorf epMot
来自美国康涅狄格大学健康中心生物医学工程系的研究人员一直在努力开发一种新的基于CRISPR的低成本诊断平台，以便检测包括新型冠状病毒SARS-CoV-2（之前称为2019-nCoV）在内的传染病。 随着近期导致2019年冠状病毒病（COVID-19）的病原体SARS-CoV-2疫情爆发，在一项
注：点击文章名称查看详情。文章名称样本仪器型号备注Assessment of the Genotoxic Potential of Azidothymidine in the Comet,Micro
ProtocolsDownloadprotocol183kbpdf?Abstract for Gel Shift Assay SystemsThe gel shift, or electrophoretic mobility shift, assay provides a simple a
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples contai
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples contai
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-