WIKI资讯
WIKI资讯
实验概要The procedure below is a general guideline procedure for ELISPOT. Abcam ELISPOT kits have been designed for detection of various cytokines and growth factors from PBMC cells (Peripheral Blood Mononuclear cells). Optimized procedures are supplied with these kits and we recommend that these methods are used.The researcher will, however, still need to optimize the cell numbers use......阅读全文
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and
21. Add 100 µL of developing buffer to all wells using a multichannel pipette and incubate at room temperature for at least 2
酶联免疫斑点技术(Enzyme Linked Immunospot Assay, ELISPOT)是细胞免疫学研究中最敏感的检测方法之一，可以在单细胞水平对抗体分泌细胞(ASC)及细胞因子(CK) 分泌细胞进行检测。由于是单细胞水平检测，ELISPOT比ELISA 和有限稀释法等具有更高的灵敏性，能
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T CellsINTRODUCTIONThe enzyme-linked immunospot (ELISPOT) assay
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable vari
主要试剂1. Blocking reagent after coating: 10% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4.2. Cells suspended in complete culture me
实验概要Cytokine ELISPOT has become a powerful routine tool for the analysis of disease- as well as vaccine-induced T-cell responses. The meth
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of prim
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of prim
五、使用仪器发表文章AuthorDateTitleJournalCell TypeCellometer / ApplicationsMahato, Ram INovember 2013Synthesis and Characterization of an Anti-Apoptotic Immu
实验概要 抗原特异性T细胞在细胞免疫应答中发挥着核心作用，因此定量测定抗原特异性T细胞数量在判定机体细胞免疫功能方面提供重要的信息。传统的抗原特异性T细胞功能检测方法有经典的Cr51释放试验、有限稀释法（LDA）、酶联免疫斑点试验（ELISPOT）
实验概要We provide a protocol using an FITC-conjugated primary antibody and a biotinylated primary antibody. Those are in turn recognized by a
实验概要 抗原特异性T细胞在细胞免疫应答中发挥着核心作用，因此定量测定抗原特异性T细胞数量在判定机体细胞免疫功能方面提供重要的信息。传统的抗原特异性T细胞功能检测方法有经典的Cr51释放试验、有限稀释法（LDA）、酶联免疫斑点试验（ELISPOT）等，但是这些方法或多或少都存在操作繁琐、检测结果误差