ELISPOTProtocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typ......阅读全文
ELISPOT Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT protocol
实验概要The procedure below is a general guideline procedure for ELISPOT. Abcam ELISPOT kits have been designed for detection of various cytokines and g
ELISPOT
随着酶联免疫分析技术在医学及生物学领域的广泛应用，使体外检测各种细胞因子及抗体研究有了新的突破。在研究免疫应答机制时以往常用用酶联免疫吸附法（ELISA）检测体液中游离的细胞因子（CK）或抗体，但由于游离的循环抗体或CK的半哀期不同，使之在体液中不断的被代谢或与靶器官结合，而不能确切的反映体内的抗
ELISPOT
实验材料 抗原试剂、试剂盒 PBSPRMI1640培养基氯化铵仪器、耗材 PVDF膜96孔板实验步骤 1. PBS 溶解抗原为30 μg/ml，加100 μl/孔于PVDF 膜铺底的96 孔灭菌板过夜； 2. 第二天吸去包被液后，加5％FCS 的PRMI 1640 培养基100 μl 封闭1 小时，
The Impact of Harmo... （一）
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and reliable a
Dual ELISPOT
实验概要We provide a protocol using an FITC-conjugated primary antibody and a biotinylated primary antibody. Those are in turn recognized by anti-FITC H
The Impact of Harmo... （二）
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable variation
ELISpot实验操作流程
ELISPOT全名为酶联免疫斑点检测，英文：Enzyme-linked Immunospot Assay。它结合了细胞培养技术与酶联免疫吸附技术（即ELISA技术），能够在单细胞水平检测细胞因子的分泌情况。其技术原理，一句话概括就是：用抗体捕获培养中的细胞分泌的细胞因子，并以酶联斑点显色的方式将其表
Immunoblot Protocol
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor