WIKI资讯
WIKI资讯
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance of tissue homeostasis and pathology, and therefore has become a hotspot of activity in biomedical fields. To date, many methods to detect apoptosis have sprung up, including morphological identification, DNA ladder observation and DNA content and cell cycle analysis. How......阅读全文
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a gi
3.6 Key references1. Kroemer G., Zamzani N., Susin S.A. Mitochondrial control of apoptosis. Immunol. Today, 18: 44-51, 1997.2. Susin S.A., Z
ANALYSIS OF CELL CYCLE Miriam Capri and Daniela Barbieri Dept. Biomedical Sciences, Sect. General Pathology,Via Campi, 287, University of Mo
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King a
美国纽约Mount Sinai 医学院 Wang, Rong（王融）教授 来自美国纽约Mount Sinai 医学院的Wang, Rong（王融）教授，做了题为“Fragmentation of Large Peptides Under Different Dissociation Conditi
第五届金属组学国际研讨会会议日程 Scientific Programme Wednesday, 9th Sept 2015 9:00 -21:00 Registration Wednesday, 9th Sept 2015, Ginkgo Hall
Beginning 1. In this paper, we focus on the need for 2. This paper proceeds as follow. 3. The structure of the paper is as follows. 4. In this
Different enzyme assays for ACTase study in H. pyloriACTase properties were studied in situ in cell-free extracts to obtain information on e
实验概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix 1.0 g of Bacto-Tryptone, 1.0 g of NaCl, 0.5 g of Yeast Extr
Introduction介绍Theoretical Framework理论概况Granular materials and fine powders are widely used in industrial applications. To control and to optimize pr
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of ChromatinLEVEL IIMaterials Bovine
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pat
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
OPERATION OF B&L SPEC. 20The most commonly encountered spectrophotometer is one manufactured by Bausch and Lomb and known as the Spec 20. The 20 r
RELATIVE CENTRIFUGAL FORCE Figure F.2. Nomogram for R.C.F.Modern day ultracentrifuges can generate forces in excess of 300,000 times that of grav
Waters推出新型SYNAPT™ G2质谱仪—动力、功能以及速度要高于任何质谱系统 即时发布 2009年6月1日 费城 Waters公司(NYSE：WAT)今天宣布推出新一代功能强大的
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role i
Measurement of ACTase activityNuclear magnetic resonance spect roscopy (NMR)The unique potential of NMR spectroscopy for monitoring simultaneously the
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a po