CriticalAppraisaloftheMTTAssayinthePresenceofRottlerin1
Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, originally described as a selective inhibitor of PKCδ, can inhibit many other PKC-unrelated kinases and has a number of biological actions, including mitochondrial uncoupling effects. We recently found that Rottlerin inhibits the transcription factor nuclear factor κB in different cell types, causing downregulation ......阅读全文
Critical Appraisal of the MTT Assay in the Presence of Rottlerin -1
Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, originally described as a selective inhibitor of PK
Critical Appraisal of the MTT Assay in the Presence of Rottlerin -2
Materials and methods Materials All chemicals and materials for cell culture (unless otherwise indicated) were obtained from Sigma (Milan, Italy).
Critical Appraisal of the MTT Assay in the Presence of Rottlerin -4
Table 1 MTT reduction normalized to cell number Time (h) MTT/cell number R5 R20 1 1.23 1.54 2 1.20 1.53
Critical Appraisal of the MTT Assay in the Presence of Rottlerin -6
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a p
Critical Appraisal of the MTT Assay in the Presence of Rottlerin -3
LDH assay LDH assay was performed in culture medium of untreated confluent cells by using a commercial kit (Sclavo Diagnostics, Siena) based on the
Critical Appraisal of the MTT Assay in the Presence of Rottlerin -5
Because an immediate sign of mitochondrial uncoupling is the drop in cell phosphorylation potential, we evaluated the Rottlerin uncoupling effect by d
MTT Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
MTT Cell Proliferation Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondri
Cytokine Bioassays
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
Hanging drop aggregation assay
DescriptionThis assay is used for the aggregation property of cancer cells. It is a very critical parameter for measurement of cell metastasis. Factor