PhosphateAssay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry the samples and standards under nitrogen evaporator (Rm 209), and add 150 ul 70% perchloric acid. Oxidize the samples and standards in a heating block (180C, Rm 218) overnight or for 1 hour if the heating block has been pre-heateed.3. Rinse the sides of the tubes with 8......阅读全文
Phosphate-Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
Phosphate-Assay-by-Suprya-Jaydev
ReagentsAshing buffer:10 g Mg(NO3)2 100 ml EtOH1.5 N HCl stock:119.7 ml concentrated HCl (11.6M @ 36% by weight)880.3 ml H2O1 N Sulfuric acid stock:28
Phosphate-(Sodium)-buffer-Chart
Phosphate (Sodium) buffer Chart Stock solution A 2 M monobasic sodium phosphate, monohydrate (276g/L) Stock solution B 2 M dibasic sodium phospha
Simplified-Calcium-Phosphate-Coprecipitation
Materials2x HEPES: 8 g NaCl, 0.105g NaHPO4, 6.5 g HEPES, H2O to 500 mL, pH 6.95 to 7.10 (try a range)2M CaCl2: store in aliquots at -20°CDNA 4 mg/mLPr
Oxidative-reactions-of-the-pentose-phosphate-pathway
One form of chemical energy used to drive biosynthetic reactions forward is the reducing power of the energy carrier NADPH. NADPH is essentia
磷酸缓冲液(phosphate-buffer)
按照下表所给定的体积,混合1 mol/L 的磷酸二氢钠(单碱)和1mol/L 磷酸氢二钠(双碱)贮液,获得所需pH的磷酸缓冲液。配制1 mol/L 的磷酸二氢钠(NaH2PO4•H2O)贮液:溶解138g于足量水中,使终体积为1L;1mol/L 磷酸氢二钠(Na2HPO4)贮液:溶解14
Pectinase-assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
Protease-assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soft
Protease-assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part
DGK-Assay
Buffers: - 2X buffer 10 ml 0.5 M imidazol, pH 6.6 0.21 g LiCl 1.25 ml 1 M MgCl2 1.0 ml 0.1 M EGTA, pH 6.6 --> Bring volume up to 50 ml with distille
TUNEL-assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Polygalacturonase-assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Aspartate-Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Bradford-Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
MTT-Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
Bradford-Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Motility-Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
Chemotaxis-Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Assay-of-Phospholipase-A-Activity
Phospholipases of the A type constitute a large family of esterases that catalyze the hydrolysis of the fatty acid ester bonds in phospholipids a
Actin-Capture-Assay
David Amberg Dialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 . Mix 5ug actin into 50ul total volume binding buffer. Mix
Needle-Assay-for-Chemotaxis
Devreotes Lab, John Hopkins Medical Institutions http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm Equipment and chemicals Zeiss
Assay-for-the-Micrococcal-Nuclease
Method: Essentially that described by Heins et al. (1966) based upon the release of acid soluble oligonucleotides following nuclease digestion of DNA
BIURET-PROTEIN-ASSAY
BIURET PROTEIN ASSAY MATERIALS Biuret Reagent Bovine serum albumin (BSA) Spectrophotometer and tubes PROCEDURE Prepare standard d
In-vitro-Sphingomyelinase-Assay
Reagents: Lysis buffer 25 mM Tris-HCl, pH 7.4 5 mM EDTA 1 mM ATP 20 µg/ml CLAP 1 mM PMSF Buffer A 10 mM MgCl2 0.2 M Tris-HCl, pH 7.4 0.2 % Triton X
ELISA-Inhibition-Assay
ELISA Inhibition AssaySensitize a 96-well microtiter plate with purified antigen.Prepare a solution of the purified antigen of interest in phosphate b
cell-proliferation-assay
cell proliferation assaybefore start:thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until c
HISTONE-KINASE-ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add:200 µg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 µL with RIPA (with pro
Glucosamine-Rapid-Assay
Glucosamine Rapid AssayMRTHOD:Place sample (containing 0.5 - 10 µg GlcN) in a Pyrex screw capped tube.Add HCl to a final concentration of 2N and a fin
Leaf-GUS-Assay
实验概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are
Bradford-protein-assay
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly