Oxidativereactionsofthepentosephosphatepathway
One form of chemical energy used to drive biosynthetic reactions forward is the reducing power of the energy carrier NADPH. NADPH is essential to drive the biosynthesis of fatty acids and cholesterol for example. While NADPH is an important energy carrier and is closely related to the high-energy electron carrier NADH, NADPH is distinct from NADH in several ways. NADH in oxidative phosphorylation in mitochondria tran......阅读全文
Oxidative－reactions－of－the－pentose－phosphate－pathway
One form of chemical energy used to drive biosynthetic reactions forward is the reducing power of the energy carrier NADPH. NADPH is essential to driv
Glycolysis－Pathway
Glycolysis was one of the first metabolic pathways studied and is one of the best understood, in terms of the enzymes involved, their mechanisms of ac
Biosynthesis－of－Chorismate－in－Bacteria－and－Plants
The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio
医学微生物学(Medical－Microbiology)词汇归纳（二）
pyrogen 致热源rabies virus 狂犬病病毒recombination 重组合，基因重组release 释放retrovirus 逆转录病毒reverse transcriptase 逆转录酶Salmonella typhi 伤寒沙门菌scarlet fever 猩红热septicem
磷酸戊糖途径
磷酸戊糖途径（pentose phosphate pathway）由6-磷酸葡萄糖开始，全过程可分为二个阶段：第一阶段是6-磷酸葡萄糖脱氢氧化生成NADPH+H 、CO2和5-磷酸核糖。第二阶段为一系列基团转移反应。
De－novo－synthesis－of－pyrimidine－nucleotides
NON-Clickable Image The pyrimidine ribonucleotides, those found in RNA, are uracil and cytosine, each containing the sugar ribose and a nitrogenous ba
Phosphate－Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
细胞代谢呼吸动态分析仪同步侦测OCR、CDPR、ECAR
北京华威中仪科技代理的由美国Seahorse Bioscience 公司最新研发的XF生物能量测定仪(细胞代谢呼吸动态分析仪)XF extracellular analyzer是世界首创使用24孔及96孔微孔盘为平台，采用无损伤ZL固态探针侦测技术即时同步侦测有氧呼吸O2(OCR)以及糖酵解作H
非结核分枝杆菌的糖分解途径有哪些酶？
非结核分枝杆菌（Non-tuberculous mycobacteria，简称NTM）是一类广泛存在于环境中的细菌，其中一些种类可以引起人类和动物的感染。在非结核分枝杆菌的基因组中，糖分解途径相关酶编码基因主要包括以下几种： 糖酵解途径（Glycolysis）：这是细胞获取能量的主要途径。非结
关于磷酸戊糖途径的基本介绍
磷酸戊糖途径（pentose phosphate pathway）是葡萄糖氧化分解的一种方式。由于此途径是由6-磷酸葡萄糖（G-6-P）开始，故亦称为己糖磷酸旁路。此途径在细胞质中进行，可分为两个阶段。 第一阶段由G-6-P脱氢生成6-磷酸葡糖酸内酯开始，然后水解生成6-磷酸葡糖酸，再氧化脱羧
什么是磷酸戊糖途径？
磷酸戊糖途径（pentose phosphate pathway）是葡萄糖氧化分解的一种方式。由于此途径是由6-磷酸葡萄糖（G-6-P）开始，故亦称为己糖磷酸旁路。此途径在细胞质中进行，可分为两个阶段。第一阶段由G-6-P脱氢生成6-磷酸葡糖酸内酯开始，然后水解生成6-磷酸葡糖酸，再氧化脱羧生成5-
Comparison－of－Beta－oxidation－in－mitochondria－and－peroxisomes－andglyoxysomes
In addition to the beta-oxidation of fatty acids in mitochondria, animal cells carry out beta-oxidation in another organelle, the peroxisome. Although
Unattended－and－Automated－Sampling－of－Chemical－Reactions
May 01, 2015 - Columbia, MDSampling chemical reactions for offline analysis by HPLC, NMR and other analytical techniques to determine reaction pro
The－reactions－that－feed－amino－groups－into－the－urea－cycle
Excess amino acids in the body can be used as a source of energy, with their carbon skeleton converted to metabolic intermediates such as acetyl-CoA o
在非结核分枝杆菌的基因组中，糖分解途径相关酶编码基因有哪些？
非结核分枝杆菌（Non-tuberculous mycobacteria，简称NTM）是一类广泛存在于环境中的细菌，其中一些种类可以引起人类和动物的感染。在非结核分枝杆菌的基因组中，糖分解途径相关酶编码基因主要包括以下几种： 糖酵解途径（Glycolysis）：这是细胞获取能量的主要途径。非结
肿瘤抑制因子p53在细胞糖代谢中的作用研究取得新成果
2月20日，国际著名学术期刊《自然—细胞生物学》（Nature Cell Biology）在线发表了中国科学技术大学生命科学学院吴缅教授和美国宾夕法尼亚大学医学院杨小鲁教授的合作研究结果：p53 regulates biosynthesis through direct inact
Phosphate－Assay－by－Suprya－Jaydev
ReagentsAshing buffer:10 g Mg(NO3)2 100 ml EtOH1.5 N HCl stock:119.7 ml concentrated HCl (11.6M @ 36% by weight)880.3 ml H2O1 N Sulfuric acid stock:28
Simplified－Calcium－Phosphate－Coprecipitation
Materials2x HEPES: 8 g NaCl, 0.105g NaHPO4, 6.5 g HEPES, H2O to 500 mL, pH 6.95 to 7.10 (try a range)2M CaCl2: store in aliquots at -20°CDNA 4 mg/mLPr
Phosphate－(Sodium)－buffer－Chart
Phosphate (Sodium) buffer ChartStock solution A2 M monobasic sodium phosphate, monohydrate (276g/L)Stock solution B2 M dibasic sodium phosphate (284 g
Cycle－Sequence－Reactions－For－Large－Insert－Plasmid－Templates
The following dye-labeled terminator reaction chemistries have been designed to balance conservation of reagents with the resulting sequence product s
概述碳同化的途径
早在十九世纪末，人们就知道光合作用需要CO2和H2O，产物是糖和淀粉，但是对于CO2是如何被还原成碳水化合物的具体步骤尚不清楚。直到20世纪40年代中期，美国加州大学的卡尔文（M.Calvin）和本森（A.Benson）采用当时的两项新技术：放射性同位素示踪和双向纸层析，以单细胞藻类作为试验材料
磷酸缓冲液（phosphate－buffer）
按照下表所给定的体积，混合1 mol/L 的磷酸二氢钠（单碱）和1mol/L 磷酸氢二钠（双碱）贮液，获得所需pH的磷酸缓冲液。配制1 mol/L 的磷酸二氢钠（NaH2PO4•H2O）贮液：溶解138g于足量水中，使终体积为1L;1mol/L 磷酸氢二钠（Na2HPO4）贮液:溶解14
The－Citric－Acid－Cycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through
Prion－Pathway
Transmissible spongiform encephalopathy (TSE) is thought to result from the structural conversion of cellular prion protein, PrP(C), into a misfolded
Dicer－Pathway
The degradation of endogenous mRNA in a sequence-specific manner can be induced by dsRNA [RNA interfernce (RNAi)], antisense transcription, or viral i
Complement－Pathway
The complement pathway consists of a series of over thirty proteins in plasma that are part of the immune response. Activation of the complement syste
Antisense－Pathway
About 8% of human genes have been estimated to carry out transcription from both DNA strands, resulting in significant level of endogenous antisense R
Fibrinolysis－Pathway
Clot formation and fibrinolysis is a balance of plasmin activation/inhibition and thrombin-thrombomodulin activity that regulates fibrin polymer forma
关于磷酸戊糖途径的概述
磷酸戊糖途径是在动、植物和微生物中普遍存在的一条糖的分解代谢途径，但在不同的组织中所占的比重不同。如动物的骨胳肌中基本缺乏这条途径，而在乳腺、脂肪组织、肾上腺皮质中，大部分葡萄糖是通过此途径分解的。在生物体内磷酸戊糖途径除提供能量外，主要是为合成代谢提供多种原料。如为脂肪酸、胆固醇的生物合成提供
Oxidative－Stress－Induced－Gene－Expression－Via－Nrf2
Reactive oxygen species (ROS) can damage biological macromolecules and are detrimental to cellular health. Electrophilic compounds, xenobiotics and an