WIKI资讯
WIKI资讯
Buffers:- 2X buffer10 ml 0.5 M imidazol, pH 6.60.21 g LiCl1.25 ml 1 M MgCl21.0 ml 0.1 M EGTA, pH 6.6--> Bring volume up to 50 ml with distilled water.- dilution buffer1 ml 0.5 M imidazol, pH 6.62.5 ml 20 mM diethylenetriaminepentaacetic acid (DTPA)--> Bring volume up to 50 ml with distilled water.Reaction Mixture:solution [stock] vol/tube # samples total vol.2X buffer - 50 µl X _______ = _______DTT 1 M 0.2 µl X......阅读全文
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable vari
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and
Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups New (Contributed by David Van Horn, Dept. of Chemistry, UC Berkeley Greg B
实验概要Alanine Transaminase (ALT) is a transaminase (EC 2.6.1.2) also called serum glutamic pyruvic transaminase (SGPT). Alanine Transaminase
实验概要The experiment provided an easy, convenient assay to measure the CoA level in a variety of biological samples. In the assay, fre
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is c
实验概要This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma
Figure 1: epMotion 5073l worktable for qPCR setup Results and DiscussionThe automation of the qPCR setup using the 10 µL dispensing tool a
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T CellsINTRODUCTIONThe enzyme-linked immunospot (ELISPOT) assay
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2A
细胞凋亡检测操作流程三--JC-1检测凋亡细胞线粒体膜电位改变凋亡检测操作流程 三、JC-1检测凋亡细胞线粒体膜电位改变BD™ MitoScreen (JC-1)线粒体膜电位检测试剂盒（551302）：组分描述JC-1(染料)4瓶，每瓶用于25个样本。冻干粉，使用前稀释至储存液
自动化TPS系列是中型开放式自动化移液工作站。一起自动化程度更高，自动更换吸头，轻松助您完成高倍率梯度稀释，单针挑样、均一化，96/384/1536孔板等移液操作，并可整合振荡器、孵育模块等功能模块。以下是美杏高德使用TPS结合微孔板筛查生物素酶的缺陷实验报告，请参考：INTRODUCTIONBio
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
Cellular Transfection and Immunoprecipitation Before proceeding with the experiments outlined below, all kinase pocket mutants should be ch
Gram Staining (+\-) (William H. Heidcamp) Gram-Staining Procedure (MEDIC, U of Texas)Very nice and detailed method description fo
DNA转染· Transfection of Mammalian Cells Using Lipofectamine (LTI)· &n
CSP-Dependent Transformation AssayTo determine if synthetic peptides activated quorum sensing for induction of genetic competence, we used the co
Or gel mobility shift assay, gel shift assay, gel retardation, electrophoretic mobility shift assay (EMSA) EMSA Using Oligos (Mike A. Dyer)A
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measur
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo
线粒体呼吸链复合物I (NADH氧化酶)、线粒体呼吸链复合物II（琥珀酸脱氢酶）是电子进入线粒体电子传递链（ETC）的主要元素。复合物I催化NADH氧化，复合物II催化琥珀酸氧化为延胡索酸。随后，辅酶Q（Q）形成辅酶（QH2），最终导致终端的电子受体O2减少。线粒体呼吸链复合物III(细胞色素c
如何借助epMotion 5073l移液工作站完成微量体积的qPCR反应体系构建在进行微量体系的液体操作时，如何避免人为误差与日间变化，如何保证结果的一致性提高实验效率？如果减少冗长枯燥的重复操作，减少昂贵试剂的使用，提升操作体验节省实验经费？本篇应用旨在介绍如何用10 μL分液工具实现全
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),