CultureofPeripheralBloodLymphocytesforChromosomeAnalysis
实验概要Provide information about chromosomal abnormalities.实验原理The blood cell karyotyping method was developed to provide information about chromosomal abnormalities. Lymphocyte cells do not normally undergo subsequent cell divisions. In the presence of a mitogen, lymphocytes are stimulated to enter into mitosis by DNA replication. After 48-72 hours, a mitotic inhibitor is added to the cult......阅读全文
Harvesting lymphocytes from Peripheral Blood
Blood Harvest· Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail· Pin animal to a board (use distal extremities) supine·
Peripheral blood “endothelial progenitor cells”
EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Human Peripheral Blood Mononuclear Cell Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fr
FACS Analysis Using Peripheral Blood Cells
FACS Analysis Using Peripheral Blood Cells Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) a
Preparation Of Peripheral Blood Cells For Chromosome Analysis
实验概要 Lymphocytes are differentiated cells which normally do not undergo subsequent cell divisions. By culturing lymphocytes in the presence of a m
Culture of Peripheral Blood Lymphocytes for Chromosome Analysis
实验概要 Provide information about chromosomal abnormalities. 实验原理 The blood cell karyotyping method was developed to provide information about chr
Isolation of peripheral blood mononuclear cells (PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3
流式应用精选——微核检测（体内&体外）
注：点击文章名称查看详情。文章名称样本仪器型号备注Assessment of the Genotoxic Potential of Azidothymidine in the Comet,Micronucleus, and Pig-a AssayBone Marrow and Peripheral
细胞组分和细胞器——染色体
Chromosomal DNA Prep : cultured cells/tissue samples (Mike A Dyer) This protocol was developed for cultured cells but should be appropriate for dis
细胞遗传学——染色体
Chromosome Staining and Banding Technique (Primate Cytogenetics Network)Protocols for different staining method, each is in great detail. Karyotype A