MTT试验方法汇总

　　Introduction

　　activation are key areas in a wide variety of cell biological approaches. The need for sensitive, quantitative, reliable and automated methods led to the development of standard assays. Such an example is based on the capability of the cells to incorporate a radioactively labeled substance ([3H])- thymidine), or to release a radioisotope such as [51Cr] after cell lysis. Alternatively, the incorporation of 5-bromo-2’-deoxyuridine (BrdU)* in place of thymidine is monitored as a parameter for DNA synthesis and cellular proliferation in immunohisto- and cytochemistry, in a cell ELISA and FACS analysis. (kits and reagents for these applications are available from Roche Molecular Biochemicals). Cell proliferation and viability assays are of particular importance for routine applications. Tetrazolium salts (e.g. MTT, XTT, WST-1) are especially useful for assaying the quantification of viable cells, because they are cleaved to form. a formazan dye (fig. 1; for UV absorbance spectrum, see fig. 2) only by metabolic active cells.