Radioiodination
Purpose and backgroundsPrinciple of radioiodinationAddition of oxidizing reagents (such as chloramine-T or peroxidase+H202) converts I- to I+ or I3-. This highly reactive molecule attacks o-position of tyrosine (or in some case, histidine is also labeled).About nuclide...125-Idecay: electron capture (g-ray, 0.035 MeV)half life: 60 daysrequires >3 mm lead plate to block >90% emission. Glass, plastic, or wat......阅读全文
Radioiodination
Purpose and backgroundsPrinciple of radioiodinationAddition of oxidizing reagents (such as chloramine-T or peroxidase+H202) converts I- to I+ or I3-.
Radioiodination－of－protein
Radioiodination (by Jun Takagi,6/16/2000)Purpose and backgroundsPrinciple of radioiodinationAddition of oxidizing reagents (such as chloramine-T or pe
A－Method－for－Assaying－Deubiquitinating－Enzymes1
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (
A－Method－for－Assaying－Deubiquitinating－Enzymes2
Table 1: Hydrolysis of 125I-labeled Ub-PESTc by the purified YUH1.Specific activity againstDUBsCbz-LRGG-AMC125I-labeled Ub-PESTcYUH13.2 x 10-105.1 x 1