Autoradiography(35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted).Make up the developer and the fixer and place in the darkroom ready for later (only if developing the same day). The developer should be a 1:1 dilution in water. The fixer is 30% (w/v) of sodium thisulphate.Place slides in a dark box and carry to dark room. Also take a slide rack, tweezers and the emulsion.Switch on ......阅读全文
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
A－quick－RNA－miniprep－for－Neurospora－mycelial－cultures
Most RNA isolation techniques currently in use have been developed for the processing of large quantities of material. These typically involve multipl
色谱术语收录大全
色谱图chromatogram；色谱峰chromatographic peak；峰底peak base；峰高h，peak height；峰宽W，peak width；半高峰宽Wh/2，peak width at halfheight；峰面积A，peak area；拖尾峰taili
A－Method－for－Assaying－Deubiquitinating－Enzymes1
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (
RNA实验方法2
13. Prehybridization mix (add in order listed at 5 mL/100 cm2 membrane)Conc.5 mL10 mL15 mLx mLStocks5x500 礚1000 礚1500 礚50x Denhardt誷5x1 mL2 mL3 m
DNA甲基化分析
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
Denaturing－Gradient－Gel－Electrophoresis－(DGGE)
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
RLGS－protocol
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants.
Cell－Cycles－－Introduction
The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes
Synthesis－and－Probing－of－Membranebound－Peptide－Arrays1
Synthesis and Probing of Membrane-bound Peptide ArraysRonald FrankDepartment of Chemical Biology, GBF (German Research Center for Biotechnology), 3812
DNA－methyltransferase－Assay
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polymerase
通过细胞受体代谢生物素化进行图像分析
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
组织学研究方法
（一）一般光学显微镜术应用一般光学显微镜（简称光镜）观察组织切片是组织学研究的最基本方法。取动物或人体的新鲜组织块，先用固定剂（fixative）固定（fixation），使组织中的蛋白质迅速凝固，防止细胞自溶和组织腐败。常用的固定剂如洒精、甲醛、醋酸、苦味酸、四氧化锇等，一般常将几种固定剂配制成混